A rapid, tag-free way to purify functional GPCRs

ABSTRACT: A GPCR purification method is described based on how the Galpha-CT (alpha5 helix) interacts with G-protein coupled receptors (GPCRs). The importance of this region in binding to and stabilizing the active GPCR state (R*) is well established, as it plays a conserved role in allosterically linking R* binding to Gα activation. Although only about 20 amino acids, the Galpha-CT contributes approximately 50% of the total interacting surface area with active receptors (R*). Galpha-CT binding also activates the G protein, by triggering conformational changes in the Galpha subunit that facilitate GDP – GTP exchange and heterotrimer dissociation.

INSTRUMENT(S):

ORGANISM(S): Cercopithecus Aethiops (green Monkey) (grivet)

SUBMITTER: Phillip Wilmarth  

LAB HEAD: David Farrens, Ph.D.

PROVIDER: PXD046070 | Pride | 2024-05-22

REPOSITORIES: Pride