Dataset Information

Protein profile of purified plasma and aqueous humor-derived extracellular vesicles from patients with ocular toxoplasmosis

ABSTRACT: To characterize extracellular vesicles proteins cargo from the aqueous humor and plasma of patients with ocular toxoplasmosis. Methods: Aqueous humor (AH) and plasma were collected from 6 patients with active ocular toxoplasmosis (OT) and 6 cataract patients (CAT). Extracellular vesicles (EVs) were isolated, and western blotting and mass spectrometry were performed for protein analysis. Results: All plasma samples from OT and CAT patients were positive for tetraspanins CD63 and TSG101 markers. However, only the AH from OT patients were positive for CD63. A total of 67 new proteins not previously reported in EVs were found in the AH and plasma from OT and CAT groups. Among these, 10 and 7 proteins were found only in the samples from CAT and OT groups, respectively. In general, these proteins were involved in immune system activation, retina homeostasis, factors related to infections and retina-associated disease. Conclusion: The distinct protein signatures between OT and CAT patients may be helpful in the differential diagnosis of OT. However, more studies are needed to better understand the role of these proteins in the pathogenesis of OT.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

SUBMITTER: Mohamed Abdouh

LAB HEAD: Julia Burnier

PROVIDER: PXD046167 | Pride | 2025-11-10

REPOSITORIES: Pride

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Protein profile of purified plasma- and aqueous humor-derived extracellular vesicles from patients with ocular toxoplasmosis.

Costa Deise F DF Pessuti Carmen Luz CL Tsering Thupten T Abdouh Mohamed M Ribeiro Kleber K Nascimento Heloisa H Commodaro Alessandra G AG Burnier Julia V JV Belfort Rubens R Burnier Miguel N MN

Arquivos brasileiros de oftalmologia 20240802 1

<h4>Purpose</h4>To characterize the extracellular vesicle protein cargo in the aqueous humor and plasma of patients with ocular toxoplasmosis.<h4>Methods</h4>Aqueous humor and plasma were collected from six patients with active ocular toxoplasmosis and six patients with cataract. Extracellular vesicles were isolated, and western blotting and mass spectrometry were performed for protein analysis.<h4>Results</h4>All plasma samples from patients with ocular toxoplasmosis and cataract were positive ...[more]

PMID: 39109736

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Project description:Glaucoma is a group of diseases characterized by progressive optic nerve damage leading to irreversible visual field loss, often associated with elevated intraocular pressure (IOP). However, the specific underlying factors or molecular mechanisms distinguishing various glaucoma subtypes are still not fully understood. In this study, we enrolled a total of 27 subjects, comprising 5 exfoliation syndrome (XFS), 4 exfoliation glaucoma (XFG), 11 primary open-angle glaucoma (POAG), and 7 cataract patients (Control) for a comparative multi-omics analysis including both proteomics and metabolomics of the aqueous humor (AH). The AH proteome and metabolome were analyzed separately using data-independent acquisition (DIA) and data-dependent acquisition (DDA) methods, after which the results were additionally integrated for a comprehensive analysis. The comparative proteomics analysis led to the identification of differentially expressed proteins (DEPs) related to lipid metabolism, complement activation, and extracellular matrix (ECM) regulation, which were commonly up-regulated in both XFG and POAG groups. The metabolomics analysis revealed differentially expressed metabolites (DEMs) particularly those involved in amino acids, suggesting their role in antioxidative processes. Thus, bioinformatic analysis suggested significant substances, such as VTN, APOA1, C6, and L-phenylalanine, that showed quantitative alterations in glaucoma patients. In addition, the integrating the individual omics analyses revealed interaction networks involving PLG, APOA1, and L-phenylalanine, or C3, APOD, and L-valine, suggesting the quantitative relationship in protein-metabolite interactions related to inflammation and lipid metabolism within the AH of glaucoma patients. Furthermore, the correlation approach identified a relationship between FGG and L-phenylalanine, and VTN and inosine, implying potential interactions among compounds not previously identified. These findings would provide valuable insights into the complex molecular mechanism differentiating glaucoma subtypes and may benefit further research into the clinical application of AH proteome and metabolome.

			Action	DRS
	CAT01_HA_1.mgf	Mgf
	CAT01_HA_1.raw	Raw
	CAT01_P_1.mgf	Mgf
	CAT01_P_1.raw	Raw
	CAT01_P_2.mgf	Mgf