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Identification of sialyl Lewisa/x-modified surface proteins in Multiple Myeloma cells by LC-MS

ABSTRACT: The project aims at identifying surface proteins that are modified by the tetrasaccharide sialyl Lewisa/x and therefore serve as selectin ligands in Multiple Myeloma. To that end, we have performed an immunoprecipitation using the Heca452 antibody (which recognizes sialyl Lewisa/x-related structures) and a matched isotype control on membrane-enriched fractions that have been generated from the sialyl Lewisa/x-enriched and parental Multiple Myeloma cell line MM1S and RPMI8226. Using this approach, we have been able to identify candidate proteins that may function as selectin ligands in myeloma.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Suspension Culture, Permanent Cell Line Cell, Plasma Cell, Cell Culture

DISEASE(S): Multiple Myeloma

SUBMITTER: Alessandro Natoni

LAB HEAD: Alessandro Natoni

PROVIDER: PXD046178 | Pride | 2024-01-23

REPOSITORIES: Pride

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