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Multi-omics analysis reveals concerted molecular responses to heat stress in a “red tide” dinoflagellate

ABSTRACT: Harmful algal blooms are induced largely by nutrient enrichment common in warm waters. An increasingly frequent phenomenon is the “red tide”: blooms of dinoflagellate microalgae that accumulate toxins lethal to other organisms in high doses. Here, we present the de novo assembled genome (~4.75 Gbp) of Prorocentrum cordatum, a globally abundant, bloom-forming dinoflagellate, and the associated transcriptome, proteome, and metabolome data from axenic cultures to elucidate the microalgal molecular responses to heat stress. We discovered, in a high-G+C genome with long introns and extensive genetic duplication, a complementary mechanism between RNA editing and exon usage that regulates dynamic expression and functional diversity of genes and proteins, and metabolic profiles that reflect reduced capacities in photosynthesis, central metabolism, and protein synthesis. These results based on multi-omics evidence demonstrate the genomic hallmark of a bloom-forming dinoflagellate, and how the complex gene structures combined with multi-level transcriptional regulation underpin concerted heat-stress responses.

INSTRUMENT(S): amaZon Speed ETD

ORGANISM(S): Prorocentrum Cordatum

SUBMITTER: Lars Wöhlbrand

LAB HEAD: Ralf Rabus

PROVIDER: PXD046193 | Pride | 2023-10-18

REPOSITORIES: Pride

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