Benchmarking and integrating human B-cell receptor genomic and antibody proteomic profiling

ABSTRACT: Antibodies are immune glycoproteins that represent a crucial part of the adaptive immune system as they mediate broad protection against viruses, bacteria, and cancer. Moreover, antibodies may serve as a unique source of information on past and current physiological and pathological events in the human body. The dysregulation of the antibody-dependent response can lead to a variety of abnormalities and diseases. Antibodies are found in the secreted form in the blood (antibody phenotype) and membrane-bound on B cells as B-cell receptor (antibody genotype). Thus, investigating the relationship between genomic and phenotypic antibody diversity is of decisive importance for understanding antibody-driven immune protection and disease. In this project, we aim to establish a robust high-throughput framework for single-cell (SC) genomic and proteomic characterization of antibody repertoires using high-throughput genomic sequencing and mass spectrometry. The generated data will be analyzed using a variety of bioinformatics tools.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Serum

SUBMITTER: Khang Le Quy  

LAB HEAD: Victor Greiff

PROVIDER: PXD046237 | Pride | 2024-06-04

REPOSITORIES: Pride