Dataset Information

Effects of Elaeagnus latifolia extract on anti-inflammation in LPS-induced macrophages

ABSTRACT: Inflammation is the essential process for responding to pathogenic infections, cancer, autoimmune diseases, and other inflammatory diseases. Oxidative stress leads to inflammatory responses. This study aims to evaluate whether the polyphenols in the Elaeagnus latifolia (EL) fruit water extract ameliorate inflammation. RAW 264.7 macrophages were stimulated with lipopolysaccharide with or without Elaeagnus latifolia (EL) extract. Quantitative PCR and ELISA were used to examine the gene expression and cytokines. Our results showed that EL extract-treated activated cells significantly decrease ROS and Nitric oxide levels by reducing the p22phox and iNOS expression and lowering cytokine-encoding genes, including IL-6, PG-E2, TNF-α relative to the LPS-activated macrophages. Furthermore, we observed the protein profiling between LPS-stimulated cell with or without EL extract. Our data establish that EL extract has antioxidant and anti-inflammatory activities and provide evidence that EL extract may be a protective compound for inflammatory diseases.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Jiradej Makjaroen

LAB HEAD: Arthid Thim-Uam

PROVIDER: PXD046383 | Pride | 2025-05-06

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

Items per page:

1 - 5 of 63

Publications

<i>Elaeagnus latifolia</i> Fruit Extract Ameliorates High-Fat Diet-Induced Obesity in Mice and Alleviates Macrophage-Induced Inflammation in Adipocytes In Vitro.

Munkong Narongsuk N Jantarach Nattanida N Yoysungnoen Bhornprom B Lonan Piyanuch P Makjaroen Jiradej J Pearngam Phorutai P Kumpunya Sarinya S Ruxsanawet Kingkarnonk K Nanthawong Saharat S Somparn Poorichaya P Thim-Uam Arthid A

Antioxidants (Basel, Switzerland) 20241205 12

<i>Elaeagnus latifolia</i> (EL) is a wild fruit known for containing several health-promoting compounds. This study aimed to evaluate the effects of EL fruit extract on high-fat diet (HFD)-induced obesity and lipopolysaccharide (LPS)-activated macrophages. Mice fed an HFD and given EL fruit extract for 10 weeks exhibited significantly lower body weight, reduced lipid accumulation, diminished oxidative stress in adipocytes, and decreased macrophage infiltration compared to those not receiving the ...[more]

PMID: 39765814

Similar Datasets

Project description:Background: Acute respiratory distress syndrome (ARDS) is characterized by refractory hypoxemia caused by accumulation of pulmonary fluid, which is related to inflammatory cell infiltration, impaired tight junction of pulmonary epithelium and impaired Na, K-ATPase function, especially Na, K-ATPase α1 subunit. Up until now, the pathogenic mechanism at the level of protein during lipopolysaccharide- (LPS-) induced ARDS remains unclear. Methods: Using an unbiased, discovery and quantitative proteomic approach, we discovered the differentially expressed proteins binding to Na, K-ATPase α1 between LPS-A549 cells and Control-A549 cells. These Na, K-ATPase α1 interacting proteins were screened by co-immunoprecipitation (Co-IP) technology. Among them, some of the differentially expressed proteins with significant performance were identified and quantified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The protein interaction network was constructed by the related Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Several differentially expressed proteins were validated by Western blot. Results: Of identified 1598 proteins, 89 were differentially expressed proteins between LPS-A549 cells and Control-A549 cells. Intriguingly, protein-protein interaction network showed that there were 244 significantly enriched co-expression among 60 proteins in the group control-A549. while the group LPS-A549 showed 43 significant enriched interactions among 29 proteins. The related GO and KEGG analysis found evident phenomena of ubiquitination and deubiquitination, as well as the pathways related to autophagy. Among proteins with rich abundance, there were several intriguing ones, including the deubiquitinase (OTUB1), the tight junction protein zonula occludens-1 (ZO-1), the scaffold protein in CUL4B-RING ubiquitin ligase (CRL4B) complexes (CUL4B) and the autophagy-related protein sequestosome-1 (SQSTM1). Conclusions: In conclusion, our proteomic approach revealed targets related to the occurrence and development of ARDS, being the first study to investigate significant differences in Na, K-ATPase α1 interacting proteins between LPS-induced ARDS cell model and control-A549 cell. These proteins may help the clinical diagnosis and facilitate the personalized treatment of ARDS.

			Action	DRS
	EL_S1F.msf	Msf
	EL_S1F1.mgf	Mgf
	EL_S1F1.raw	Raw
	EL_S1F10.mgf	Mgf
	EL_S1F10.raw	Raw