Project description:isoTOP-ABPP performed for a small library of cysteine-reactive compounds to assess cysteine ligandability of viral and host proteins towards these compounds.
Project description:proteomics experiments performed for the elucidation of compound induced PDIA6 interactions by AP-MS and label free quantifiaction experiments to assess compound dependent UPR activation
Project description:Seeds of the double mutant opr3-3 coi1-1 were germinated in Johnson media supplemented with 0.5 µM coronatine, to select homozygous coi1-1 plants, at 21°C under long-day conditions (16-h light/8-h dark)
Project description:Algal discs were transferred to a 12-well plate containing 2 ml liquid 0.5 Gamborg’s B5 mock or OPDA 25 µM, incubated for 2 hours on a shaker (120 rpm) and then the algae were frozen in liquid nitrogen and ground to a fine powder.