Proteome of Corynebacterium glutamicum at different pH values

ABSTRACT: Recently, we established Corynebacterium glutamicum as a suitable production host for various bacteriocins including garvicin Q (GarQ). Here, we establish secretion of GarQ by C. glutamicum via the Sec translocon. At neutral pH, the cationic peptide is efficiently adsorbed to the negatively charged envelope of producer bacteria limiting availability of the bacteriocin in culture supernatants. Using a reporter strain and proteomic analyses, we identified HtrA, a protease associated with secretion stress, as another potential factor limiting GarQ production.

INSTRUMENT(S):

ORGANISM(S): Corynebacterium Glutamicum Atcc 13032

SUBMITTER: Valentin Steier  

LAB HEAD: Marco Oldiges

PROVIDER: PXD046499 | Pride | 2024-01-08

REPOSITORIES: Pride