Extracellular vesicules proteomic profile of P. brasiliensis yeasts recovered from granulomatous lesions of C57BL/6 WT mice

ABSTRACT: C57BL/6 WT mice were infected with 1x10^6 P. brasiliensis yeasts. After 8 weeks and 12 weeks of infection the animals were euthanized and the granulomatous lesions were recovered. Then, the yeasts were recovered and cultivated in a rich medium. After cultivation the yeasts extracellular vesicles were isolated and their proteins extracted, digested with trypsin and analyzed by LC-MS/MS. The data show an induced metabolism by the recovered yeasts, with a higher abundance of proteins related to energy metabolism, lipid metabolism, nucleotide metabolism, amino acid metabolism. Additionally there is a higher abundance of some virulence factor.

INSTRUMENT(S):

ORGANISM(S): Paracoccidioides Brasiliensis (strain Pb18)

SUBMITTER: Bruno Borges  

LAB HEAD: Flávio Vieira Loures

PROVIDER: PXD046549 | Pride | 2024-05-23

REPOSITORIES: Pride