Dataset Information

Caspase-4 and Caspase-5 Coordinate the Sensing of Streptococcus pyogenes Extracellular vesicles

ABSTRACT: Caspase-4 and caspase-5 have been linked to the sensing of Gram-negative bacteria and their extracellular vesicles (EVs). Recent studies suggested that Gram-positive bacteria can be sensed through signaling cascades involving these two caspases. In the present study, we report that S. pyogenes secretes vesicles decorated with LTA on their surface that can activate caspase-4/-5 and the NLRP3 inflammasome. We show that this sensing process depends on particle internalization and Toll-like receptor (TLR) signaling. Finally, we demonstrate that caspase-4/-5 participate in the recognition of EVs from other Gram-positive bacteria.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Kathirvel Alagesan

LAB HEAD: Emmanuelle Charpentier

PROVIDER: PXD046570 | Pride | 2025-10-20

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20250212_150159_24C021_Ves-depleted_T2_Report.tsv	Tabular
	21C003_results_2nd.genes.tsv	Tabular
	E1_241220_24C021_Crude_DIA.raw	Raw
	E1_241220_24C021_EVs_UZ_DIA.raw	Raw
	E1_241220_24C021_Ves-depleted_SUP_DIA.raw	Raw

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Publications

Streptococcus pyogenes EVs induce the alternative inflammasome via caspase-4/-5 in human monocytes.

Krause Kathrin K Franch Arroyo Sandra S Ugolini Matteo M Kueck Tonya T Sullivan Timothy J TJ Gálvez Eric J C EJC Muenzner Matthias M Goosmann Christian C Brinkmann Volker V Frese Christian K CK Alagesan Kathirvel K Vierbuchen Tim T Heine Holger H Resch Ulrike U Sander Leif E LE Charpentier Emmanuelle E

EMBO reports 20250908 19

The sensing of Gram-negative Extracellular Vesicles (EVs) by the innate immune system has been extensively studied in the past decade. In contrast, recognition of Gram-positive EVs by innate immune cells remains poorly understood. Comparative genome-wide transcriptional analysis in human monocytes uncovered that S. pyogenes EVs induce proinflammatory signatures that are markedly distinct from those of their parental cells. Among the 209 genes exclusively upregulated by EVs, caspase-5 prompted us ...[more]

PMID: 40925957

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