Proteomics

Dataset Information

0

The ER stress sensor IRE1 interacts with STIM1 to promote store-operated calcium entry, T cell activation, and muscular differentiation

ABSTRACT: A quantitative phosphoproteomic study was performed on mouse embryonic fibroblasts (MEF) knocked-out of IRE1 protein and re rexpressin it culturen in 25mM glucose media. The chosen experimental strategy was to perform phosphopeptide enrichment associated with multiplex protein identification and quantification by LC-MSMS on a high-resolution mass spectrometer using tandem mass tag (TMT9; Thermo Fisher Scientific) technology and High- Select Fe-NTA Phosphopeptide Enrichment Kit (Thermo Fisher Scientific).

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Embryonic Fibroblast, Fibroblast

SUBMITTER: Amado Carreras  

LAB HEAD: Amado Carreras Sureda

PROVIDER: PXD046579 | Pride | 2023-12-08

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
20221216_LU50HMC35_pl22119_AC_TMT10_P.raw Raw
20221216_LU50HMC37_pl22119_AC_TMT10_HpH_F01.raw Raw
20221216_LU50HMC38_pl22119_AC_TMT10_HpH_F02.raw Raw
20221216_LU50HMC39_pl22119_AC_TMT10_HpH_F03.raw Raw
20221216_LU50HMC40_pl22119_AC_TMT10_HpH_F04.raw Raw
Items per page:
1 - 5 of 21
altmetric image

Publications

The ER stress sensor IRE1 interacts with STIM1 to promote store-operated calcium entry, T cell activation, and muscular differentiation.

Carreras-Sureda Amado A   Zhang Xin X   Laubry Loann L   Brunetti Jessica J   Koenig Stéphane S   Wang Xiaoxia X   Castelbou Cyril C   Hetz Claudio C   Liu Yong Y   Frieden Maud M   Demaurex Nicolas N  

Cell reports 20231205 12

Store-operated Ca<sup>2+</sup> entry (SOCE) mediated by stromal interacting molecule (STIM)-gated ORAI channels at endoplasmic reticulum (ER) and plasma membrane (PM) contact sites maintains adequate levels of Ca<sup>2+</sup> within the ER lumen during Ca<sup>2+</sup> signaling. Disruption of ER Ca<sup>2+</sup> homeostasis activates the unfolded protein response (UPR) to restore proteostasis. Here, we report that the UPR transducer inositol-requiring enzyme 1 (IRE1) interacts with STIM1, promote  ...[more]

Similar Datasets

Transcriptomics Urinary exosome microRNA signatures as a non-invasive prognostic biomarker for prostate cancer
2021-06-07 | GSE173094 | GEO
Genomics Thermo Fisher Scientific OncoScan expression array data for undifferentiated uterine sarcomas (CNV)
2019-08-23 | GSE119042 | GEO
Transcriptomics Thermo Fisher Scientific OncoScan expression array data for undifferentiated uterine sarcomas (expression)
2019-08-23 | GSE119041 | GEO
Transcriptomics CIRCULATING SERUM MICRO-RNA AS NON-INVASIVE DIAGNOSTIC BIOMARKERS OF ENDOMETRIOSIS
2024-10-30 | GSE279435 | GEO
Transcriptomics Elucidating the Role of the Desmosome Protein p53 Apoptosis Effector Related to PMP-22 in Growth Hormone Tumors
2022-09-29 | GSE214226 | GEO
Genomics The regulons of an OmpR-like transcription factor in Pseudomonas syringae pv. Actinidiae
2022-12-13 | GSE220275 | GEO
Genomics Thermo Fisher Scientific OncoScan expression array data for undifferentiated uterine sarcomas
| PRJNA487811 | ENA
Genomics MicroRNA-138 Suppresses Glioblastoma Proliferation through Downregulation of CD44 (NanoString)
2021-03-01 | GSE165937 | GEO
Genomics Thermo Fisher Scientific OncoScan expression array data for undifferentiated uterine sarcomas (expression)
| PRJNA487812 | ENA
Genomics Thermo Fisher Scientific OncoScan expression array data for undifferentiated uterine sarcomas (CNV)
| PRJNA487813 | ENA