Analysis of conformational changes induced by binding of allosteric nanobodies to a bacterial LRRK2 homologue by CX-MS

ABSTRACT: As part of an in-depth characterization of nanobodies directed against the Chlorobium tepidum protein CtRoco, a bacterial LRRK2 homologue, chemical crosslinking combined with mass spectrometry (CX-MS) was performed. Two allosteric nanobodies binding to two different epitopes in the LRR and the Roc domain of CtRoco, respectively were analysed using the CID-cleavable crosslinker disuccinimidyl sulfoxide (DSSO).

INSTRUMENT(S):

ORGANISM(S): Chlorobium Tepidum

SUBMITTER: Christian Johannes Gloeckner  

LAB HEAD: Christian Johannes Gloeckner

PROVIDER: PXD046634 | Pride | 2024-04-23

REPOSITORIES: Pride