Analysis of conformational changes induced by binding of allosteric nanobodies to a bacterial LRRK2 homologue by CX-MS
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ABSTRACT: As part of an in-depth characterization of nanobodies directed against the Chlorobium tepidum protein CtRoco, a bacterial LRRK2 homologue, chemical crosslinking combined with mass spectrometry (CX-MS) was performed. Two allosteric nanobodies binding to two different epitopes in the LRR and the Roc domain of CtRoco, respectively were analysed using the CID-cleavable crosslinker disuccinimidyl sulfoxide (DSSO).
INSTRUMENT(S): Orbitrap Fusion
ORGANISM(S): Chlorobium Tepidum
SUBMITTER: Christian Johannes Gloeckner
LAB HEAD: Christian Johannes Gloeckner
PROVIDER: PXD046634 | Pride | 2024-04-23
REPOSITORIES: Pride
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