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Pilot evaluation of long-term reproducibility of capillary zone electrophoresis-tandem mass spectrometry for top-down proteomics of a complex proteome sample

ABSTRACT: In this work, for the first time, we performed a pilot investigation of long-term reproducibility of CZE-MS for TDP of a complex sample (i.e., a yeast cell lysate) to achieve a better understanding of advantages, issues and potential solutions of CZE-MS for large-scale TDP.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Candida Albicans (yeast) Saccharomyces Cerevisiae

SUBMITTER: Liangliang Sun

LAB HEAD: Liangliang Sun

PROVIDER: PXD046651 | Pride | 2024-03-20

REPOSITORIES: Pride

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Pilot Evaluation of the Long-Term Reproducibility of Capillary Zone Electrophoresis-Tandem Mass Spectrometry for Top-Down Proteomics of a Complex Proteome Sample.

Sadeghi Seyed Amirhossein SA Chen Wenrong W Wang Qianyi Q Wang Qianjie Q Fang Fei F Liu Xiaowen X Sun Liangliang L

Journal of proteome research 20240228 4

Mass spectrometry (MS)-based top-down proteomics (TDP) has revolutionized biological research by measuring intact proteoforms in cells, tissues, and biofluids. Capillary zone electrophoresis-tandem MS (CZE-MS/MS) is a valuable technique for TDP, offering a high peak capacity and sensitivity for proteoform separation and detection. However, the long-term reproducibility of CZE-MS/MS in TDP remains unstudied, which is a crucial aspect for large-scale studies. This work investigated the long-term q ...[more]

PMID: 38417052

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