Dataset Information

The extracellular matrix protein signatures of the outer and inner zone of the rat adrenal cortex

ABSTRACT: Different sites and changes in the tissue microenvironment have been associated with the cortex of adrenal gland functionality, maintenance, and renewal. Here we describe the differences between the extracellular matrix (ECM) signatures of the outer (OF) and inner fractions (IF) of the rat adrenal cortex. We compared the proteomic profiles of decellularized OF and IF rat adrenal samples followed by the analysis of the proteomic profiles of male and female samples. Our analysis of OF and IF samples simultaneously showed a total of 186 proteins, 69 proteins were identified in OF and 144 in IF. Ninety-two proteins were in common to both fractions, 5 proteins were exclusive to OF, and 89 proteins were exclusive to IF. A total of 42 proteins were assigned as ECM, 13 as structural ECM constituents, 17 as ECM components, and 12 as related to functional processes in ECM. Col14a1, Fbn2, and Ltbp4 were exclusive to and upregulated in the OF while Gpt2, Chga, Chgb, and Plg were exclusive to and upregulated in the IF. In addition, Nid2 and Lama2, both ECM components, were identified as upregulated in female OF and male IF adrenal, respectively. Next, we validated the main findings by using qPCR, histochemistry, and immunohistochemistry approaches.

INSTRUMENT(S):

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Adrenal Gland

SUBMITTER: Veronica Santiago

LAB HEAD: Claudimara Ferini

PROVIDER: PXD046828 | Pride | 2024-08-09

REPOSITORIES: Pride

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Extracellular Matrix Protein Signatures of the Outer and Inner Zones of the Rat Adrenal Cortex.

Kremer Jean Lucas JL Santiago Veronica Feijoli VF Bongiovani Rodrigues Fernanda F Auricino Thais Barabba TB Freitas Danilo Henriques de Oliveira DHO Palmisano Giuseppe G Lotfi Claudimara Ferini Pacicco CFP

Journal of proteome research 20240717 8

This study analyzes the extracellular matrix (ECM) signatures of the outer (OF = capsule + subcapsular + zona glomerulosa cells) and inner fractions (IF = zona fasciculata cells) of the rat adrenal cortex, which comprise two distinct microenvironment niches. Proteomic profiles of decellularized OF and IF samples, male and female rats, identified 252 proteins, with 32 classified as ECM-component and ECM-related. Among these, 25 proteins were differentially regulated: 17 more abundant in OF, inclu ...[more]

PMID: 39018382

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