Dataset Information

Methodological aspects of µLC-MS/MS for wide-scale proteomic analysis of anthracycline-induced cardiomyopathy

ABSTRACT: The effort to utilize microflow liquid chromatography for deep-scale proteomic analysis (µLC-MS/MS) is still growing. In this work, we utilized two-dimensional LC separation and peptide derivatization by tandem mass tag and assessed the capability of µLC-MS/MS to reveal protein changes associated with severe anthracycline-induced cardiomyopathy phenotype in comparison with nLC-MS/MS. Finally, control and rabbit myocardium specimens with experimentally induced anthracycline cardiomyopathy were analysed. Nearly 4000 myocardial proteins were quantified using µLC-MS/MS. In concordance with nLC-MS/MS, data evaluation revealed that severe anthracycline cardiotoxicity phenotype was associated with marked global protein dysregulation. Observed protein dysregulation consisted of profound remodelling of cardiomyocyte sarcomere as well as components of extracellular matrix. Pro-inflammatory proteins and multiple antioxidant proteins were markedly changed, whereas the levels of mitochondrial proteins were declined compared to control myocardium. In this study, µLC-MS/MS was confirmed as a versatile proteomic tool for comprehensive mapping of proteome changes related to anthracycline cardiotoxicity having a great potential to become full-fledged alternative to nLC.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Oryctolagus Cuniculus (rabbit)

TISSUE(S): Heart

SUBMITTER: Rudolf Kupcik

LAB HEAD: Marie Vajrychova

PROVIDER: PXD047014 | Pride | 2025-04-30

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	210403_Oryctolagus_cuniculus_uniprot_proteome_UP000001811.fasta	Fasta
	Myocardium_Daunorubicin_TMT_6samples_Nano_vs_Micro_OxP_mqpar.xml	Xml
	RK049_2DTMT_6S_A_10ug_01.raw	Raw
	RK049_2DTMT_6S_B_10ug_01.raw	Raw
	RK049_2DTMT_6S_CH_10ug_01.raw	Raw

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Methodological Aspects of μLC-MS/MS for Wide-Scale Proteomic Analysis of Anthracycline-Induced Cardiomyopathy.

Kupčík Rudolf R Lenčová Olga O Mazurová Yvona Y Štěrba Martin M Vajrychová Marie M

ACS omega 20250318 12

The efforts to utilize microflow liquid chromatography hyphenated to tandem mass spectrometry (μLC-MS/MS) for deep-scale proteomic analysis are still growing. In this work, two-dimensional LC separation and peptide derivatization by a tandem mass tag (TMT) were used to assess the capability of μLC-MS/MS to reveal protein changes associated with the severe chronic anthracycline cardiotoxicity phenotype in comparison with nanoflow liquid chromatography (nLC-MS/MS). The analysis of the control and ...[more]

PMID: 40191338

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