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4D label-free acetylation-modified quantitative proteome of mouse FGSCs.

ABSTRACT: This study aims to determine the changes in related acetylation-modified proteins after FGSCs treatment.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture

SUBMITTER: Hong Zhou

LAB HEAD: Ji Wu

PROVIDER: PXD047214 | Pride | 2025-05-06

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	MS_identified_information.txt.zip	Txt
	RE041LPAc_C1_Slot1-34_1_9018.d.zip	Other
	RE041LPAc_C2_Slot1-35_1_9020.d.zip	Other
	RE041LPAc_T1_Slot1-36_1_9022.d.zip	Other
	RE041LPAc_T2_Slot1-37_1_9024.d.zip	Other

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Publications

Nicotinamide mononucleotide promotes female germline stem cell proliferation by activating the H4K16ac-Hmgb1-Fyn-PLD signaling pathway through epigenetic remodeling.

Zhou Hong H Liu Yujie Y Tian Geng G GG Wu Ji J

Cell & bioscience 20250417 1

<h4>Background</h4>Nicotinamide mononucleotide (NMN), an endogenous nucleotide essential for various physiological processes, has an unclear role and regulatory mechanisms in female germline stem cell (FGSC) development.<h4>Results</h4>We demonstrate that NMN significantly enhances FGSC viability and proliferation. Quantitative acetylation proteomics revealed that NMN markedly increases the acetylation of histone H4 at lysine 16 (H4K16ac). Subsequent chromatin immunoprecipitation sequencing (ChI ...[more]

PMID: 40247362

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4D label-free quantitative proteome of mouse FGSCs

Project description:Identification of differentially expressed proteins following treatment of FGSCs.

2025-05-06 | PXD047216 | Pride

Ventral hippocampal CA1 modulates pain behaviors in mice with peripheral inflammation

Project description:Chronic pain is one of the most significant and costly medical problems throughout the world. Recent evidence has confirmed the hippocampus as an active modulator of pain chronicity but the underlying mechanisms remain poorly defined. By means of in vivo electrophysiology together with chemogenetic and optogenetic manipulations in freely behaving mice, we identified a neural ensemble in the ventral hippocampal CA1 (vCA1) that showed inhibitory responses to noxious external stimuli, but not to innocuous stimuli. Following peripheral inflammation, this neuronal ensemble became responsive to innocuous stimuli and causally contributed to sensory hypersensitivity in inflammatory animals. Mimicking this inhibition of vCA1 neurons using chemogenetics in naïve mice induced chronic pain-like behavioral changes, whereas activating these vCA1 neurons in mice with chronic peripheral inflammation resulted in a striking reduction of pain-related behaviors. Pathway-specific manipulation of vCA1 projections to the basolateral amygdala (BLA) and infralimbic cortex (IL) showed that these pathways were differentially involved in pain modulation at different temporal stages of chronic inflammatory pain. These results confirm a crucial role of the ventral hippocampus and its circuits in modulating the development of chronic pain in mice.

2022-12-19 | PXD038416 | Pride

Global lysine acetylome analysis of Picea asperata somatic 1 embryos during partial desiccation

Project description:By the combination of affinity enrichment and high-resolution LC-MS/MS analysis, large-scale lysine acetylome analysis was performed in picea asperata. Altogether, 1,360 lysine acetylation sites in 717 protein groups were identified. Intensive bioinformatic analysis was then carried out to annotate those lysine acetylated targets, including protein annotation, functional classification, functional enrichment, etc.

2018-10-26 | PXD005042 | Pride

Quantitative analysis of the global proteome of heart tissue in septic shock

Project description:Nowadays, sepsis and septic shock have become major public health problems, which are the main cause of death among patients admitted to the intensive care units. Notably, myocardial dysfunction during sepsis, usually called sepsis-induced myocardial depression, is common in septic shock patients whose incidence is about 70% and leads to a high mortality. However, the mechanism underlying the septic myocardial depression is still unclear. Recently, proteomics has become a powerful method for explore protein dynamics and their complex regulatory mechanism, and thus generates a profound impact on precision medicine and the clinical setting. Importantly, the expression patterns of global proteins in heart tissue between sepsis and control group remain unclear. Therefore, we performed the rat models of sepsis-induced myocardial depression and investigated global protein expression profiles in heart tissue between sepsis and control group using 4D label-free proteomic technique.

2025-05-19 | PXD029649 | Pride

E3 ubiquitin ligase Listerin regulates macrophage cholesterol efflux and atherosclerosis by targeting ABCA1

Project description:Atherosclerosis arises from disrupted cholesterol metabolism, notably impaired macrophage cholesterol efflux leading to foam cell formation. Through single-cell and bulk RNA sequencing, we identified Listerin as a regulator of macrophage cholesterol metabolism. Listerin expression increased during atherosclerosis progression in humans and rodents. Its deficiency suppressed cholesterol efflux, promoted foam cell formation, and exacerbated plaque features (macrophage infiltration, lipid deposition, necrotic cores) in macrophage-specific knockout mice. Conversely, Listerin overexpression attenuated these atherosclerotic manifestations. Mechanistically, Listerin stabilizes ABCA1, a key cholesterol efflux mediator, by catalyzing K63-linked polyubiquitination at residues K1884/K1957, countering ESCRT-mediated lysosomal degradation of ABCA1 induced by oxLDL. ABCA1 agonist Erythrodiol restored cholesterol efflux in Listerin-deficient macrophages, while ABCA1 knockout abolished Listerin's effects in THP-1 cells. This study establishes Listerin as a protective factor in atherosclerosis via post-translational stabilization of ABCA1, offering a potential therapeutic strategy targeting ABCA1 ubiquitination to enhance cholesterol efflux.

2025-06-11 | PXD064532 | Pride

LSM14B is essential for oocyte meiotic maturation by regulating maternal mRNA storage and clearance

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2023-10-17 | PXD042761 | Pride

Tuina reverses capsule fibrosis in Frozen Shoulder as indicated via phosphoproteomics

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2024-01-09 | PXD047468 | Pride

Tuina reverses capsule fibrosis in Frozen Shoulder as indicated via protemocis

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2024-01-09 | PXD047467 | Pride

Proteomic Comparison Between Hyphae and Spores Reveals Pathogenicity Diversity in Mucor Irregularis

Project description:Mucor irregularis poses significant clinical risks, including severe disfigurement, with hyphal forms being more commonly encountered in clinical settings compared to spore forms. However, the pathogenic differences between these forms at the protein level remain poorly understood. This study employed TMT-based quantitative proteomics to investigate the proteomic differences between spores and hyphae of Mucor irregularis. Our analysis revealed that differentially abundant proteins are predominantly expressed during the hyphal stage. Notably, key enzymes such as RhoGEF GTPase, Subtilisin-related Protease, Acyl-CoA Synthetase, and Ribonuclease T2 appear crucial for hyphal morphological development and pathogenic mechanisms. Additionally, myosins play a significant role not only in hyphal growth and virulence but also in influencing sensitivity to antifungal agents. These findings provide new insights into the morphogenesis, development, and metabolic processes of Mucor irregularis, laying a foundation for further research into its pathogenic mechanisms.

2025-07-28 | PXD055430 | Pride

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2024-05-24 | PXD042935 | Pride