Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Affinity purification MS of human Ubiquitin-tagged RIT1

ABSTRACT: Human RIT1 was tagged with HA tag + N or C terminal ubiquitin (HA-Ubiqutin-RIT1 and HA-RIT1-Ubiqutin) and compared with empty vector (EV) or S35N/M90I RIT1 mutant (HA-RIT1 S35N/M90I). The constructs were expressed in human 293T cells and affinity purified using HA beads. RIT1 interacting proteins were compared by quantifying MS1 extracted chromatograms of peptides identified in conventional target-decoy database search.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Anatoly Urisman

LAB HEAD: Anatoly Urisman, MD PhD

PROVIDER: PXD047228 | Pride | 2024-07-09

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	Pau_2021-05-16.blib	Other
	Pau_2021-05-16.txt	Txt
	Z20210516-02.raw	Raw
	Z20210516-02.txt	Txt
	Z20210516-03.raw	Raw

Items per page:

1 - 5 of 39

Similar Datasets

Wells Lab Primary T Cell Surfaceomics

Project description:Glycoprotein cell surface capture proteomics of effector T cells following activation as well as co-culture with Tregs and hypoxic culture.

2022-03-03 | PXD024789 | Pride

ChIP-chip to determine the regulation of the K. lactis hsgs (ChIP of MATa1, MATalpha2, and RME1)

Project description:ChIP-chip to determine the regulation of the K. lactis hsgs (ChIP of MATa1, MATalpha2, and RME1). The MATa1 and MATalpha2 ChIPs were performed in an a/alpha cell using N-terminally HA-tagged proteins and the RME1 ChIPs were perfomed in an a cell using C-terminally myc-tagged protein. For the RME1 ChIPs, the cells grown with out phosphate. For the MATa1 andMATalpha2 ChIPs the cells were grown in YEPD. Epitope tagged strains were compared to untagged control strains. Two biological replicates were preformed for the RME1 ChIP. For the MATa1 and MATalpha2 ChIP, peaks were considered indicative of binding if both MATa1 and MATalpha2 showed enrichment above the untagged control.

2010-12-01 | E-GEOD-25209 | biostudies-arrayexpress

RNA binding profile of MED23 in 293T cells

Project description:To delineate the repertoire of RNA binding of MED23, we performed a denatured CLIP-seq experiment coupled with high-salt wash in MED23-HA 293T cells and wildtype 293T cells as a control.

2025-05-28 | GSE211081 | GEO

HA-tagged FOXM1 isoforms

Project description:HA-tagged FOXM1 isoforms

| PRJEB24594 | ENA

HA-tagged ROCK2 plasmid

Project description:HA-tagged ROCK2 plasmid

| PRJEB24608 | ENA

Genomic occupation profile of Mediator MED23 in 293T cells

Project description:We performed CUT&Tag experiments in MED23-HA and WT 293T cells to analyze the genomic occupation profiles for the Mediator MED23.

2025-05-28 | GSE211082 | GEO

GNPS - PAP-gamma associates with PAXT nuclear exosome to control the stability of PROMPT ncRNAs

Project description:Endogenous ZFC3H1 was tagged in 293T cells. Dignam cellular extracts where subjected to sequential FLAG and HA immunoprecipitation and elution. Complexes were then analyzed using mass spectrometry at the taplin mass spectrometry facility. The 2 samples are FLA-HA IP in wild-type 293T cells and Flag-HA IP in FH-ZFC3H1 293T cells.

2022-05-16 | MSV000089482 | GNPS

Protein interactions with HA-tagged cytoplasmic dynein-2 DATASET2

Project description:To identify those proteins interacting with the cytoplasmic dynein-2 using mammalian cell lines stably expressing HA-tagged intermediate chain subunits, WDR34 (DYNC2I2) or WDR60 (DYNC2I1).

2023-01-24 | PXD031153 | Pride

Protein interactions with HA-tagged cytoplasmic dynein-2 DATASET1

2023-01-24 | PXD031151 | Pride

Protein interactions with HA-tagged cytoplasmic dynein-2 DATASET1

2023-01-24 | PXD031154 | Pride