Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Global proteomic analysis for pellet of Chlamydomonas reinhardtii for parental strain and DYRKP KO mutant

ABSTRACT: We want to know the diversity of proteomes in parental strain and DYRKP KO mutant.

INSTRUMENT(S):

ORGANISM(S): Chlamydomonas Reinhardtii

TISSUE(S): Plant Cell, Cell Culture

SUBMITTER: MINJAE KIM

LAB HEAD: Yonghua Li-Beisson

PROVIDER: PXD047255 | Pride | 2025-09-01

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	137AH_Pellet_1_1239.d.zip	Other
	137AH_Pellet_2_1241.d.zip	Other
	137AH_Pellet_3_1242.d.zip	Other
	137AH_Pellet_4_1243.d.zip	Other
	137AH_Pellet_5_1244.d.zip	Other

Items per page:

1 - 5 of 15

Publications

Increasing lipid accumulation in Chlamydomonas by serial knocking out of DYRKP1 kinase and ADP-glucose pyrophosphorylase.

Kim Minjae M Kim Ji Yeon JY Han Kyong Ha KH Shin Hyeon Ho HH Jin EonSeon E

Microbial cell factories 20250822 1

Microalgae are promising sustainable feedstocks for biodiesel production. Among the primary carbon reservoirs in microalgae, starch and lipids are the main targets for metabolic engineering aimed at enhancing productivity. Redirecting carbon flux from starch toward lipid biosynthesis has been considered an effective strategy to improve lipid yield, and manipulating upstream regulators may allow broader control over metabolic networks. DYRKP1, a plant-specific dual-specificity tyrosine-phosphoryl ...[more]

PMID: 40846950

Similar Datasets

Knocking out the carboxyltransferase interactor 1 (CTI1) in Chlamydomonas boosted oil content by fivefold without affecting cell growth

Project description:Acetyl-CoA carboxylase (ACCase) catalyzes the first and rate-limiting step in de novo fatty acid synthesis in the chloroplast. ACCase activity has thus been subjected to positive and negative regulations. Among negative regulators, one finds the pII, BADC and the carboxyltransferase interactor (CTI) in Arabidopsis thaliana. However, we know little about the regulation of fatty acid synthesis in algae. In this study, we identified only one homolog of the plant CTI in Chlamydomonas and show that the Chlamydomonas CrCTI1 indeed interacts with the Chlamydomonas alhpa-carboxyltransferase (CT) ACCase subunit by yeast two-hybrid protein-protein interaction assay. The Chlamydomonas CrCTI1 knocked-out mutants, obtained from the CRISPR-Cas9 mutagenesis, accumulated higher amount of total fatty acids and stored more triacylglycerols (TAGs) in lipid droplets, without affecting membrane lipids. The TAG phenotype of the crcti1 mutants was not influenced by light, but rather is affected by trophic styles. Moreover, cell cultivation under heterotrophic conditions in darkness revealed the crucial function of CrCTI1 in homeostasis between lipid accumulation and cell growth. Comparative proteomics analysis of the crcti1 mutants and WT suggested a role for CrCTI1 in maintaining carbon flux and redox balance under different carbon availability. Taken together, this study reveals the occurrence and function of a negative regulator of fatty acid synthesis in algae and provides a molecular brick that can be used for genetic engineering of microalgae for biotechnology purposes.

2025-02-26 | PXD053580 | Pride

Transcriptome analysis of insecticide resistant housefly strain: insights about SNPs and regulatory elements in differentially expressed cytochrome P450 genes

Project description:The 791spin is the spinosad-selected strain derived from 791a, a laboratory strain derived from a multi-resistant field-collected sample of houseflies. The 791a strain proved highly resistant to pyrethroids and some anticholinesterases and showed some resistance to the chitin synthesis-disrupting larvicides.In order to understand the evolution of insecticide resistance, de novo assembly of a spinosad resistant housefly strain 791spin using 454 technology was initiated Transcriptome analysis of insecticide resistant housefly strain compared to susceptible strain

2015-05-12 | E-GEOD-65891 | biostudies-arrayexpress

Gene Expression Defines Natural Changes in Mammalian Lifespan

Project description:Mammals differ more than hundred fold in maximum lifespan, which can be altered in either direction during evolution, but the molecular basis for natural changes in longevity is not understood. Divergent evolution of mammals also led to extensive changes in gene expression within and between lineages. To understand the relationship between lifespan and variation in gene expression, we carried out RNA-seq-based gene expression analyses of liver, kidney and brain of 33 diverse species of mammals. Our analysis uncovered parallel evolution of gene expression and lifespan, as well as the associated life history traits, and identified the processes and pathways involved. These findings provide direct insights into how Nature reversibly adjusts lifespan and other traits during adaptive radiation of lineages. RNA-seq gene expression profiling in normal liver, kidney and brain of 33 mammalian species.

2015-02-17 | E-GEOD-43013 | biostudies-arrayexpress

De novo assembled databases allow species-specific protein-based stable isotope probing of microbiomes without prior knowledge of the community composition

Project description:Dataset generated and used in "De novo assembled databases allow species-specific protein-based stable isotope probing of microbiomes without prior knowledge of the community composition". For more information, see SDRF.sdrf.tsv

2025-09-08 | PXD057215 | Pride

Histone post-translational modifications of Trypanosoma cruzi: a high-confidence comprehensive map

Project description:In humans and other eukaryotes, histone post-translational modifications (hPTMs) play an essential role in the epigenetic control of gene expression. In trypanosomatid parasites, conversely, gene regulation occurs mainly at the post-transcriptional level. However, our group has recently shown that hPTMs are abundant and varied in Trypanosoma cruzi, the etiological agent of Chagas Disease, signaling for possible conserved epigenetic functions. Here, we provide a high-confidence comprehensive map of hPTMs, distributed in all canonical, variant and linker histones of T. cruzi. Our updated landscape expands the number of known T. cruzi hPTMs by almost 2-fold, representing the largest dataset of hPTMs available to any trypanosomatid to date, and can be used as a basis for functional studies on the dynamic regulation of chromatin by epigenetic mechanisms and the selection of candidates for the development of epigenetic drugs in trypanosomatids.

2021-01-04 | PXD019104 | Pride

Developmental gene expression of Hymenochirus boettgeri

Project description:RNA-seq experiments to construct Hymenochirus bettgeri transcripts Collect mRNA from whole eggs/embryos/tadpoles, with poly-A enrichment method

2016-10-24 | E-GEOD-76089 | biostudies-arrayexpress

Identification of myristoylated proteins in zebrafish (Danio rerio)

Project description:Multi-functional capture reagents reported here enable robust identification of metabolically-tagged myristoylated proteomes with unprecedented confidence resulting from the combination of chemical probe-based enrichment, and release and direct detection of lipid-modified peptides by MS. Whilst capture reagents containing enzymatically cleavable linkers have been previously reported they typically require an extra proteolytic step and their capacity to enable detection of lipidated peptides has not been demonstrated. Herein, we report the largest database (85 counts) of experimentally validated human proteins that are myristoylated at an endogenous level in living cells. Furthermore, we demonstrate the first profile of myristoylation in a living multicellular organism and the confident identification of over 50 novel targets. Importantly, this is also the first example of analysis of any protein lipidation event during development. Our methodology is novel in analytical/chemical approach, and provides quantitative and dynamic information. This submission concerns myristoylated proteomes of zebrafish origin.

2015-03-30 | PXD001876 | Pride

Next Generation Protein Sequencing

Project description:NGPS is a method for de-novo, full-length protein sequencing in high throughput. The method is based on cleavage of the protein at semi-random sites by microwave-assisted acid hydrolysis (MAAH), enrichment of LC-MS/MS amenable peptides from the hydrolysate by solid-phase-extraction, LC-MS/MS analysis, de-novo long peptide tag sequencing of resulting peptides and assembly of peptide tags into consensus contigs.

2017-04-03 | PXD003804 | Pride

Mass spectrometry of Manica rubida venom

Project description:In the present study, the venom peptidome of the red ant, Manica rubida, was characterized using an integrated transcriptomic and proteomic approach. In addition, insecticidal assays on C18 reversed-phase HPLC venom fractions were conducted on Lucilia caesar blowflies to identify neurotoxic peptides.

2020-03-20 | PXD015675 | Pride

Nesocodon mauritianus nectar proteins

Project description:The purpose of this project was to identify the nectar proteins of Nesocodon mauritianus

2021-11-04 | PXD029394 | Pride