Compound-centric chemical proteomics to screen fructose binding proteins

ABSTRACT: To identify protein targets of fructose, we performed a compound-centric chemical proteomics (CCCP) experiment on podocytes with P-fructose. Briefly, podocytes were incubated with P-fructose, and the cellular proteins that directly bind to fructose were covalently crosslinked to P-fructose by UV irradiation. After orthogonal conjugation was achieved with azide-biotin via click chemistry and affinity purification was performed, the P-fructose-enriched proteins were analyzed by liquid chromatography coupled with tandem mass spectrometry (LC‒MS/MS).

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Kidney

SUBMITTER: Jingzi Zhang  

LAB HEAD: Jingzi Zhang

PROVIDER: PXD047310 | Pride | 2026-06-05

REPOSITORIES: Pride