Human 293T cells lysosomal osmotic stress response
Ontology highlight
ABSTRACT: The goal of this project is to identify changes of the lysosomal surface proteome upon the induction of lysosomal vacuolation. 293T cells stably expressing LAMP1-GFP-TurboID or Lyso-TurboID were treated with a small molecule apilimod or DMSO to trigger lysosomal vacuolation, after which the lysosomal surface proteins were labeled with biotin by TurboID. The biotinylated proteins by Lyso-TurboID were purified by streptavidin beads for mass spectrometry.
INSTRUMENT(S):
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Cell Culture
SUBMITTER:
Jay Xiaojun Tan
LAB HEAD: Jay Xiaojun Tan, PhD
PROVIDER: PXD047404 | Pride | 2025-08-21
REPOSITORIES: Pride
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