Proteomics

Dataset Information

0

Human 293T cells lysosomal osmotic stress response


ABSTRACT: The goal of this project is to identify changes of the lysosomal surface proteome upon the induction of lysosomal vacuolation. 293T cells stably expressing LAMP1-GFP-TurboID or Lyso-TurboID were treated with a small molecule apilimod or DMSO to trigger lysosomal vacuolation, after which the lysosomal surface proteins were labeled with biotin by TurboID. The biotinylated proteins by Lyso-TurboID were purified by streptavidin beads for mass spectrometry.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Jay Xiaojun Tan  

LAB HEAD: Jay Xiaojun Tan, PhD

PROVIDER: PXD047404 | Pride | 2025-08-21

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
MSB66000ABand_01.raw Raw
MSB66000ABand_01__F199598_.mzid.gz Mzid
MSB66000ABand_01__F199598_.mzid_MSB66000ABand_01__F199598_.MGF Mzid
MSB66000ABand_02.raw Raw
MSB66000ABand_02__F199599_.mzid.gz Mzid
Items per page:
1 - 5 of 61

Similar Datasets

2012-12-10 | E-GEOD-35453 | biostudies-arrayexpress
2012-12-10 | E-GEOD-35500 | biostudies-arrayexpress
2015-02-09 | E-GEOD-64172 | biostudies-arrayexpress
2021-09-23 | PXD027476 | Pride
2022-09-07 | PXD028852 | Pride
2023-07-26 | PXD027545 | Pride
2025-04-22 | PXD054968 | Pride
2018-06-07 | PXD007547 | Pride
2012-01-10 | E-GEOD-34954 | biostudies-arrayexpress
2020-04-20 | PXD015243 | Pride