Human 293T cells lysosomal osmotic stress response

ABSTRACT: The goal of this project is to identify changes of the lysosomal surface proteome upon the induction of lysosomal vacuolation. 293T cells stably expressing LAMP1-GFP-TurboID or Lyso-TurboID were treated with a small molecule apilimod or DMSO to trigger lysosomal vacuolation, after which the lysosomal surface proteins were labeled with biotin by TurboID. The biotinylated proteins by Lyso-TurboID were purified by streptavidin beads for mass spectrometry.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Jay Xiaojun Tan  

LAB HEAD: Jay Xiaojun Tan, PhD

PROVIDER: PXD047404 | Pride | 2025-08-21

REPOSITORIES: Pride