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The chromatin factors SET-26 and HCF-1 oppose the histone deacetylase HDA-1 in longevity and gene regulation in C. elegans

ABSTRACT: In this study, a GFP-tagged version of the protein HCF-1 was immuno-precipitated from C. elegans lysates of the strain IU171 and co-purifying proteins were identified by LC-MS/MS. As a negative control, the same immuno-precipitation was conducted from wild-type (strain N2) worms that did not carry a HCF-1::GFP transgene.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Caenorhabditis Elegans

TISSUE(S): Whole Body

SUBMITTER: Christian Riedel

LAB HEAD: Christian G. Riedel

PROVIDER: PXD047509 | Pride | 2024-01-29

REPOSITORIES: Pride

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The chromatin factors SET-26 and HCF-1 oppose the histone deacetylase HDA-1 in longevity and gene regulation in <i>C. elegans</i>.

Emerson Felicity J FJ Chiu Caitlin C Lin Laura Y LY Riedel Christian G CG Zhu Ming M Lee Siu Sylvia SS

bioRxiv : the preprint server for biology 20230323

SET-26, HCF-1, and HDA-1 are highly conserved chromatin factors with key roles in development and aging. Here we present mechanistic insights into how these factors regulate gene expression and modulate longevity in <i>C. elegans</i>. We show that SET-26 and HCF-1 cooperate to regulate a common set of genes, and both antagonize the histone deacetylase HDA-1 to limit longevity. We propose a model in which SET-26 recruits HCF-1 to chromatin in somatic cells, where they stabilize each other at the ...[more]

PMID: 36993207

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