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Identification of the FTO O-GlcNAcylation sites by LC-MS/MS

ABSTRACT: We determined the O-GlcNAcylation sites of FTO by LC-MS/MS.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture

SUBMITTER: Lu Zhang

LAB HEAD: Xiao-Lian Zhang

PROVIDER: PXD047698 | Pride | 2025-07-14

REPOSITORIES: Pride

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			Action	DRS
	1.p	Other
	2.p	Other
	Search_files.zip	Other
	YS-600-FTO-Ni.raw	Raw
	YS-600-FTO.raw	Raw

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Publications

FTO O-GlcNAcylation promotes TRIM21-mediated FTO ubiquitination degradation to sustain the negative feedback control of macrophage inflammation.

Zhang Lu L Liu Min M Xie Yan Y Yuan Bi-Feng BF Peng Zhiyong Z Xiong Jun J Zhang Xiao-Lian XL

Frontiers in immunology 20250626

<h4>Introduction</h4>The fat mass and obesity-associated protein (FTO), a key RNA N<sup>6</sup>-methyladenosine (m<sup>6</sup>A) demethylase, has been highlighted for its important role in inflammatory response. Emerging evidences link the O-GlcNAcylation to numerous human diseases, particularly inflammation. However, the specific role and underlying mechanism of FTO O-GlcNAcylation in inflammation remain elusive.<h4>Methods</h4>The FTO O-GlcNAcylation modification was determined by co-immunopre ...[more]

PMID: 40642069

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FTO over expression in mice

Project description:In this study, we aimed to examine the changes in gene expression that occur in FTO over expressing mice in order to gain more insight into the underlying mechanisms by which FTO influences body weight and adiposity

2014-06-01 | E-MTAB-2331 | biostudies-arrayexpress

Comparison of phosphorylation status of Wnt proteins in control and Fto deficient cells upon Wnt 3a stimulation

Project description:Wnt Phospho protein profiling comparing control and Fto deficient cell after Wnt3a stimulation Control vs Fto deficient MEFs treated with Wnt 3a condioned medium for 40 minutes. 6 replicates per array

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FTO: gene expression and function in skeletal muscle

Project description:We identified the target genes of FTO ("fat mass and obesity associated") in primary cultures of human skeletal muscle cells using adenoviral vectors expressing FTO or GFP and oligonucleotide microarrays. Human myotubes were prepared from 4 different skeletal muscle biopsies. After differentiation, myotubes were infected for 48 hours with recombinant adenovirus expressing either GFP or FTO. Each FTO-infected myotubes culture was compared to GFP-infected myotubes culture. GFP-infected myotubes were regarded as the control. Four biological replicates were processed.

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Effect of FTO overexpression and knockdown on transcriptome of HEK293 cells

Project description:To gain insight into FTO function, we knocked down and overexpressed FTO in HEK293 cells.Genetrail analyses of expression profiles pointed to the RNA splicing and processing machinery. Intriguingly, using immunocytochemistry and confocal laser scanning microscopy, we observed strong enrichment of FTO in nuclear speckles and - to a lesser extent - in nucleoli, but not in other known nuclear bodies. We also studied RNA samples of Fto knockout and wild type mice with regard to content of methylated and unmethylated nucleosidesand observed that ratios of modified and unmodified uracil and adenine were different depending on the presence of FTO. Taken together, our data suggest that FTO is involved in RNA processing and modification. We used microarrays to investigate global gene expression changes depending on the level of FTO We compared FTO overexpressing and FTO depleted cells to cells with endogeneus level of FTO to determine global gene expression changes.

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Mass spetrometry analysis of FTO-interacting proteins

Project description:We identified the proteins which interact with fat mass and obesity-associated protein (FTO).

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A lncRNA from the FTO locus acts as an inhibitor of the m6A writer complex and p53 tumor suppression signaling

Project description:control RNA and FTO-IT1 RNA and their interacting proteins were pulled down by biotin-streptavidin beads to see the interacting protein of FTO-IT1 RNA.

2024-08-09 | PXD041053 | Pride

Next-generation sequencing for the fat mass and obesity-associated protein (FTO) binding mRNA

Project description:Purpose: The goals of this study are to investigate the mRNAs that bind to the FTO protein in the white fat tissue from mice. Methods: 1. White fat from CAG promoter driven transgenic Flag-tagged FTO mice were extracted with RNA protected. 2. Flag-FTO proteins were immunoprecipitated with control IP using IgG. 3. The immunoprecipitated RNAs were deep sequenced and analyzed. Results: We focused on the mRNAs which have functions related to lipid metabolism and homeostasis. Immunoprecipitated RNAs profiles from Flag-FTO IP and IgG IP were generated by deep sequencing.

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The Obesity-associated Fto gene regulates activity of the dopaminergic midbrain circuitry [FTO KO]

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2013-06-15 | E-GEOD-47216 | biostudies-arrayexpress

The Obesity-associated Fto gene regulates activity of the dopaminergic midbrain circuitry [WT]

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