Dataset Information

GST Pull-down-MS identify candidate host factors that interact with CHIKV nsP4

ABSTRACT: The Chikungunya virus (CHIKV) is a single positive-stranded RNA virus transmitted by mosquitoes. More than 40 countries have reported cases of CHIKV infection to varying degrees. Currently, there is no licensed specific antiviral drug or vaccine for CHIKV in the world. In this study, we have identified host protein interactors of CHIKV nsP4 proteins in 293T cells and the major cellular pathways involved in the interaction between host factors and CHIKV nsp4 using Co-Immunoprecipitation and GST Pull-down followed by high-throughput quantitative mass spectrometry.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Disease Free

SUBMITTER: Yao Yan

LAB HEAD: Yan Yao

PROVIDER: PXD047753 | Pride | 2025-05-06

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	YAS202308010077_1_GST_RDRP1.msf	Msf
	YAS202308010077_1_GST_RDRP1.raw	Raw
	YAS202308010077_1_GST_RDRP2.msf	Msf
	YAS202308010077_1_GST_RDRP2.raw	Raw
	YAS202308010077_1_NC.msf	Msf

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Publications

Identification of RACK1 as a novel regulator of non-structural protein 4 of chikungunya virus.

Yan Yao Y Zhang Fengyuan F Zou Meng M Chen Hongyu H Xu Jingwen J Lu Shuaiyao S Liu Hongqi H

Acta biochimica et biophysica Sinica 20240501 10

Chikungunya virus (CHIKV) is a neglected arthropod-borne and anthropogenic alphavirus. Over the past two decades, the CHIKV distribution has undergone significant changes worldwide, from the original tropics and subtropics regions to temperate regions, which has attracted global attention. However, the interactions between CHIKV and its host remain insufficiently understood, which dampens the need for the development of an anti-CHIKV strategy. In this study, on the basis of the optimal overexpre ...[more]

PMID: 38813597

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