Name: Chemoproteomic profiling to identify Ophiobolin A targets
Keywords: Ophiobolin

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Chemoproteomic profiling to identify Ophiobolin A targets

ABSTRACT: Cysteine and lysine chemoproteomic profiling of Ophiobolin A

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Lung Endothelial Cell

DISEASE(S): Lung Carcinoma

SUBMITTER: Daniel Nomura

LAB HEAD: Daniel Nomura

PROVIDER: PXD047763 | Pride | 2023-12-12

REPOSITORIES: Pride

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Ophiobolin A Covalently Targets Complex IV Leading to Mitochondrial Metabolic Collapse in Cancer Cells.

Gowans Flor A FA Thach Danny Q DQ Wang Yangzhi Y Altamirano Poblano Belen E BE Dovala Dustin D Tallarico John A JA McKenna Jeffrey M JM Schirle Markus M Maimone Thomas J TJ Nomura Daniel K DK

bioRxiv : the preprint server for biology 20230309

Ophiobolin A (OPA) is a sesterterpenoid fungal natural product with broad anti-cancer activity. While OPA possesses multiple electrophilic moieties that can covalently react with nucleophilic amino acids on proteins, the proteome-wide targets and mechanism of OPA remain poorly understood in many contexts. In this study, we used covalent chemoproteomic platforms to map the proteome-wide reactivity of OPA in a highly sensitive lung cancer cell line. Among several proteins that OPA engaged, we focu ...[more]

PMID: 36945520

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