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A natural small molecule alleviates liver fibrosis by targeting apolipoprotein L2 (APOL2)-mediated endoplasmic reticulum (ER) stress

ABSTRACT: To investigate the functional role of APOL2 in liver fibrosis, we performed an unbiased immunoprecipitation-mass spectrometry (IP-MS) analysis for APOL2 in the TGF-β1-stimulated LX-2 cell lysates.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Lu Gan

LAB HEAD: Sheng Yin

PROVIDER: PXD047815 | Pride | 2024-08-13

REPOSITORIES: Pride

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			Action	DRS
	APOL2-1.raw	Raw
	APOL2-2.raw	Raw
	APOL2-3.raw	Raw
	APOL2VSIgG-1.msf	Msf
	APOL2VSIgG-2.msf	Msf

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A natural small molecule alleviates liver fibrosis by targeting apolipoprotein L2.

Gan Lu L Jiang Qiwei Q Huang Dong D Wu Xueji X Zhu Xinying X Wang Lei L Xie Wei W Huang Jialuo J Fan Runzhu R Jing Yihang Y Tang Guihua G Li Xiang David XD Guo Jianping J Yin Sheng S

Nature chemical biology 20240805 1

Liver fibrosis is an urgent clinical problem without effective therapies. Here we conducted a high-content screening on a natural Euphorbiaceae diterpenoid library to identify a potent anti-liver fibrosis lead, 12-deoxyphorbol 13-palmitate (DP). Leveraging a photo-affinity labeling approach, apolipoprotein L2 (APOL2), an endoplasmic reticulum (ER)-rich protein, was identified as the direct target of DP. Mechanistically, APOL2 is induced in activated hepatic stellate cells upon transforming growt ...[more]

PMID: 39103634

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