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DiGly_Analysis_RAD18_RAD6_with/without_MAGEA4

ABSTRACT: Auto-ubiquitination assay performed in which the E3 ligase complex RAD18-RAD6 was incubated with E1 and ubiquitin in the presence/absence of MAGEA4for 1 hour at 37C. All proteins were purified from E.coli. Samples were loaded and run onto a precast SDS PAGE gel, which was stained with Coomasie. Approximately 20ug of RAD18-RAD6 was used for the assay.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Frank Stein

LAB HEAD: Sagar Bhogaraju

PROVIDER: PXD047850 | Pride | 2024-02-20

REPOSITORIES: Pride

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