Dataset Information

Total Synthesis and Target Identification of Marine Cyclopiane Diterpenes

ABSTRACT: We proceeded to competitive chemoproteomic profiling with conidiogenone C probe in LPS-stimulated RAW264.7 cell lysates. The lysis was then reacted with azide-biotin, and after enrichment and digestion, the peptides were labeled as “light, “medium” or “heavy” by dimethyl labeling. The labeled proteins were identified and quantified by LC-MS/MS analysis.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Macrophage

SUBMITTER: shan jiang

LAB HEAD: Houhua Li

PROVIDER: PXD047981 | Pride | 2025-05-06

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20221028_2647_lysate_CC2_3dimethyl_1020.raw	Raw
	20221028_2647_lysate_CC2_3dimethyl_1020_combined_averaged_ratios.txt	Txt
	20221028_2647_lysate_CC2_3dimethyl_1025.raw	Raw
	20221028_2647_lysate_CC2_3dimethyl_1025_combined_averaged_ratios.txt	Txt
	20230504_2647_lysate_CCP_dimethy_20230430_03.raw	Raw

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Publications

Total synthesis and target identification of marine cyclopiane diterpenes.

Li Tian T Jiang Shan S Dai Yuanhao Y Wu Xia X Guo Huihui H Shi Liang L Sang Xueli X Ren Li L Wang Jie J Shi Lili L Zhou Wenming W Li Houhua H Hao Hong-Dong HD

Nature communications 20241230 1

Marine cyclopianes are a family of diterpenoid with novel carbon skeleton and diverse biological activities. Herein, we report our synthetic and chemical proteomics studies of cyclopiane diterpenes which culminate in the asymmetric total synthesis of conidiogenones C, K and 12β-hydroxy conidiogenone C, and identification of Immunity-related GTPase family M protein 1 (IRGM1) as a cellular target. Our asymmetric synthesis commences from Wieland-Miescher ketone and features a sequential intramolecu ...[more]

PMID: 39738095

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