Total Synthesis and Target Identification of Marine Cyclopiane Diterpenes

ABSTRACT: We proceeded to competitive chemoproteomic profiling with conidiogenone C probe in LPS-stimulated RAW264.7 cell lysates. The lysis was then reacted with azide-biotin, and after enrichment and digestion, the peptides were labeled as “light, “medium” or “heavy” by dimethyl labeling. The labeled proteins were identified and quantified by LC-MS/MS analysis.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Macrophage

SUBMITTER: shan jiang  

LAB HEAD: Houhua Li

PROVIDER: PXD047981 | Pride | 2025-05-06

REPOSITORIES: Pride