LC-MS/MS after Immunoprecipitation with Mbd5 in zebrafish at 4dpf

ABSTRACT: To address how Mbd5 binding to m5C RNAs mediates transcriptional activation of its target genes. Using the Tg[zhsp70l:FLAGmbd5iso2-E2AGFP] zebrafish, we performed heat shock at multiple developmental stages and carried out co-immunoprecipitation (co-IP) at 4 dpf using the FLAG antibody, followed by mass spectrometry

INSTRUMENT(S):

ORGANISM(S): Danio Rerio (zebrafish) (brachydanio Rerio)

TISSUE(S): Embryo

SUBMITTER: Jianhua Guo  

LAB HEAD: Su Guo

PROVIDER: PXD048128 | Pride | 2024-06-16

REPOSITORIES: Pride