Dataset Information

MUT-7 exoribonuclease activity and localisation are mediated by an ancient domain

ABSTRACT: The MUT-7 family of 3’-5’ exoribonucleases is evolutionarily conserved across the animal kingdom and plays essential roles in small RNA production in the germline. Most MUT-7 homologs carry a C-terminal domain of unknown function named MUT7 C appended to the exoribonuclease domain. Our analysis shows that the MUT7-C is evolutionary ancient, as a minimal version of the domain exists as an individual protein in prokaryotes. Throughout evolution, MUT7-C has acquired different insertions in animals, expanding its functions. C. elegans MUT-7 contains a specific insertion within MUT7-C, which allows binding to MUT 8 and, consequently, MUT-7 recruitment to germ granules. In addition, in C. elegans and human MUT-7, the MUT7 C domain contributes to RNA binding and is thereby crucial for nuclease activity. This RNA-binding function most likely represents the ancestral function of the MUT7 C domain. Overall, this study sheds light on MUT7-C and assigns two functions to this previously uncharacterised domain.

INSTRUMENT(S):

ORGANISM(S): Escherichia Coli Caenorhabditis Elegans

TISSUE(S): Cell Culture

SUBMITTER: WeiQiang Chen

LAB HEAD: Sebastian Falk

PROVIDER: PXD048205 | Pride | 2024-10-17

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20220221_E3_RSLC3_Busetto_Falk_MPL_UniWien_XL_truncated_30p.raw	Raw
	MaxQquant_txt.zip	Other
	Merox_result.zip	Other
	Pride-submission_Raw_file_experiment_list.xlsx	Xlsx
	checksum.txt	Txt

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Publications

MUT-7 exoribonuclease activity and localization are mediated by an ancient domain.

Busetto Virginia V Pshanichnaya Lizaveta L Lichtenberger Raffael R Hann Stephan S Ketting René F RF Falk Sebastian S

Nucleic acids research 20240801 15

The MUT-7 family of 3'-5' exoribonucleases is evolutionarily conserved across the animal kingdom and plays essential roles in small RNA production in the germline. Most MUT-7 homologues carry a C-terminal domain of unknown function named MUT7-C appended to the exoribonuclease domain. Our analysis shows that the MUT7-C is evolutionary ancient, as a minimal version of the domain exists as an individual protein in prokaryotes. In animals, MUT7-C has acquired an insertion that diverged during evolut ...[more]

PMID: 39188014

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