Dataset Information

TRPV2 ion channel interactome reveals functional interaction with NCAM and L1CAM in neurite outgrowth

ABSTRACT: TRPV2 voltage-insensitive, calcium-permeable ion channels play important roles in cancer progression, immune response, and neuronal development. Despite TRPV2’s physiological impact, underlying endogenous proteins mediating TRPV2 responses and affected signaling pathways remain elusive. Using quantitative peroxidase-catalyzed (APEX2) proximity proteomics we uncover dynamic changes in the TRPV2-proximal proteome and identify calcium signaling and cell adhesion factors recruited to the molecular channel neighborhood in response to activation. Quantitative TRPV2 proximity proteomics further revealed activation-induced enrichment of protein clusters with biological functions in neural and cellular projection. We demonstrate a functional connection between TRPV2 and the neural immunoglobulin cell adhesion molecules NCAM and L1CAM. NCAM and L1CAM stimulation robustly induces TRPV2 [Ca2+]I flux in neuronal PC12 cells and this TRPV2-specific [Ca2+]I flux requires activation of the protein kinase PKC. TRPV2 expression directly impacts neurite lengths that are modulated by NCAM or L1CAM stimulation. Hence, TRPV2’s calcium signaling plays a previously undescribed, yet vital role in cell adhesion and TRPV2 calcium flux and neurite development are intricately linked via NCAM and L1CAM cell adhesion proteins.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Gary Bradshaw

LAB HEAD: Vera Moiseenkova-Bell

PROVIDER: PXD048282 | Pride | 2025-07-14

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	554456_PG_May2022_APEX_18plex_Fr2_13724_psc_20ppm.mzIdentML	Mzid
	554499_PG_May2022_APEX_18plex_Fr3_13725_psc_20ppm.mzIdentML	Mzid
	554501_PG_May2022_APEX_18plex_Fr6_13728_psc_20ppm.mzIdentML	Mzid
	554503_PG_May2022_APEX_18plex_Fr5_13727_psc_20ppm.mzIdentML	Mzid
	554505_PG_May2022_APEX_18plex_Fr4_13726_psc_20ppm.mzIdentML	Mzid

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Publications

The dynamic TRPV2 ion channel proximity proteome reveals functional links of calcium flux with cellular adhesion factors NCAM and L1CAM in neurite outgrowth.

Gallo Pamela N PN Mihelc Elaine E Eisert Robyn R Bradshaw Gary A GA Dimek Florian F Leffler Andreas A Kalocsay Marian M Moiseenkova-Bell Vera V

Cell calcium 20240504

TRPV2 voltage-insensitive, calcium-permeable ion channels play important roles in cancer progression, immune response, and neuronal development. Despite TRPV2's physiological impact, underlying endogenous proteins mediating TRPV2 responses and affected signaling pathways remain elusive. Using quantitative peroxidase-catalyzed (APEX2) proximity proteomics we uncover dynamic changes in the TRPV2-proximal proteome and identify calcium signaling and cell adhesion factors recruited to the molecular c ...[more]

PMID: 38728789

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