Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Idenfication the receptor on KRAS p.G12D cell that interact with FN1859

ABSTRACT: To capture the unknown receptors, we purified recombinant His-tagged FN1859 proteins from Escherichia coli and performed His-tag pull-down assays with WT, KRAS p.G13D and KRAS p.G12D cell lysates. Then the bands submitted for mass spectrometry (MS) protein identification.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Colon

SUBMITTER: Huiyuan Zhu

LAB HEAD: Huanlong Qin

PROVIDER: PXD048684 | Pride | 2024-01-25

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Similar Datasets

Identification of the cellular component interacted with F. nucleatum.

Project description:We incubated F. nucleatum lysates with biotinylated proteins from KRAS p.G12D, KRAS p.G13D and KRAS WT cells respectively. Subsequent western blot analysis showed that three candidate proteins from F. nucleatum might interact with KRAS p.G12D cells. The bands of interest were then submitted for mass spectrometry (MS) protein identification, which reported three proteins.

2024-01-25 | PXD048686 | Pride

Mass spectrometry (MS) datasets of PURPL RNA pulldown samples in A375 cells

Project description:We performed PURPL RNA pulldown followed by MS analysis to identify purpl-interacting proteins in A375 cells.With high performance liquid chromatography-mass spectrometry (HPLC-MS), the binding protein of PURPL from RNA pull down was detected by removing non-specific protein which were binding with EGFP.

2022-02-16 | PXD028863 | Pride

LncRNA LINC01186 directly bingding proteins in Hepatocellular carcinoma

Project description:We conducted an RNA pulldown assay in Hepatocellular carcinoma cell line HepG2 using in vitro-transcribed full-length LINC01186 RNA accompanied with a control EGFP RNA. The specific binding proteins of LINC01186 was identified using high-performance liquid chromatography-mass spectrometry (HPLC-MS).

2022-07-23 | PXD031844 | Pride

EP300 promotes ferroptosis via HSPA5 acetylation in pancreatic cancer

Project description:Ferroptosis is a form of regulated cell death characterized by oxidative injury-induced lipid peroxidation. However, the detailed protein post-translational modification regulatory mechanism of ferroptosis remains largely unknown. Here, we report that E1A binding protein P300 (EP300) acetyltransferase promotes ferroptosis in human pancreatic ductal adenocarcinoma (PDAC) cells via the acetylation of heat shock protein family A (Hsp70) member 5 (HSPA5, also known as GRP78 or BIP) on the site of K353. Acetylated HSPA5 loses its ability to inhibit lipid peroxidation and subsequent ferroptotic cell death. Genetic or pharmacological inhibition of EP300-mediated HSPA5 acetylation on K353 increases PDAC cell resistance to ferroptosis. Moreover, histone deacetylase 6 (HDAC6) limits HSPA5 acetylation and subsequent ferroptosis.

2023-10-24 | PXD039998 | Pride

human, gastric cancer line, LC-MSMS

Project description:We also tested the downstream differentiation of protein expression between BGC823 Ctrl and BGC823 MIR137HG by IP target FUS following LC-MS/MS. And we selected several proteins related to tumor growth and metastasis to analyze further. String data showed that they might have more contact with each other. Western blot data showed that MET, CTNNB1 and RHOC were also co-expressed with the over-expression MIR137HG.

2022-08-12 | PXD031258 | Pride

renal cancer cell line ACHN LC-MSMS

Project description:To predict the protein that interact with FOXA2 and illustrate the important role and the biological mechanism it may involved of FOXA2 in renal cancer.

2024-01-26 | PXD043802 | Pride

Transcription profiling of human breast cell lines and one normal RNA sample

Project description:Total RNA from seven breast cell lines and one normal RNA samples was labelled using the Illumina TotalPrep RNA Amplification kit (Ambion) following manufacturer's instructions. 1.5 ug of biotin-labelled cRNA were used for each hybridisation on Sentrix Human-6 BeadChips (Illumina, San Diego, CA) following manufacturer's protocol.

2007-11-17 | E-TABM-194 | biostudies-arrayexpress

Transcription profiling of human breast cancer cell lines

Project description:Total RNA from 46 breast cell lines was labelled using the Illumina TotalPrep RNA Amplification kit (Ambion) following manufacturerï¿½s instructions. 1.5 ï¿½g of biotin-labelled cRNA were used for each hybridisation on Sentrix Human-6 v1 BeadChips (Illumina, San Diego, CA) following manufacturerï¿½s protocol.

2011-11-11 | E-TABM-827 | biostudies-arrayexpress

Haemonchus contortus ESPs LS-MS/MS

Project description:Haemonchus contortus has developed complexed and multifaceted mechanisms of immune evasion to enable the survival in the host. Generating excretion and secretion products (ESPs) to subvert or suppress the functions of host cytokines is a newly immune regulatory pattern found during recent years. TGF-β1 has critical immune regulatory functions in nematode infections. In this study, the co-immunoprecipitation was used to collect the molecules which bound to the goat TGF-β1 from ESPs, and the precipitates were identified by mass spectrometry using shotgun LC-MS / MS.

2020-09-08 | PXD020699 | Pride

the Interaction Proteome of Ribosomal 40S Components PNO1 and NOB1 Using TurboID Proximity Labeling Technology

Project description:Background: The ribosome assembly factors PNO1 and NOB1 play crucial roles in the maturation of the 40S ribosomal small subunit. TurboID is an efficient biotin ligase that can biotinylate proteins in proximity to the target protein and is widely used to study complex biological processes within cells.Here, we utilized this technology to investigate the complex interaction network of PNO1 and NOB1 within cells.

2024-06-08 | PXD052825 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data