Autophagosome content profiling in TBK1-E696K knockin MEFs

ABSTRACT: To determine how the mutant TBK1-E696K protein impacts autophagosomes, we performed autophagosome content profiling using protease protection coupled APEX2 proximity proteomics of autophagosomes of homozygous TBK1-E696K knockin and wiltype mouse embryonic fibroblasts (MEFs) transfected with a APEX2-LC3 as previously described in Zellner et al. 2021 Molecular Cell.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Embryo, Fibroblast

DISEASE(S): Huntington Disease

SUBMITTER: Christian Behrends  

LAB HEAD: Christian Behrends

PROVIDER: PXD048795 | Pride | 2024-03-16

REPOSITORIES: Pride