Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Release of immunogenic peptides from precursor protein CspD

ABSTRACT: Identification of immunogenic peptides from Pseudomonas protein CspD digested by apoplastic fluids (AFs) from Nicotiana benthamiana.

INSTRUMENT(S):

ORGANISM(S): Nicotiana Benthamiana Pseudomonas Syringae Pv. Tomato Str. Dc3000

SUBMITTER: Farnusch Kaschani

LAB HEAD: Farnusch Kaschani

PROVIDER: PXD048912 | Pride | 2025-06-23

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ACE_0686-02_CC03.raw	Raw
	ACE_0686-02_CC04.raw	Raw
	ACE_0686-02_CC09.raw	Raw
	ACE_0686-02_CC10.raw	Raw
	ACE_0686_CC03.raw	Raw

Items per page:

1 - 5 of 12

Publications

Extracellular plant subtilases dampen cold-shock peptide elicitor levels.

Chen Changlong C Buscaill Pierre P Sanguankiattichai Nattapong N Huang Jie J Kaschani Farnusch F Kaiser Markus M van der Hoorn Renier A L RAL

Nature plants 20241011 11

Recognizing pathogen-associated molecular patterns on the cell surface is crucial for plant immunity. The proteinaceous nature of many of these patterns suggests that secreted proteases play important roles in their formation and stability. Here we demonstrate that the apoplastic subtilase SBT5.2a inactivates the immunogenicity of cold-shock proteins (CSPs) of the bacterial plant pathogen Pseudomonas syringae by cleaving within the immunogenic csp22 epitope. Consequently, mutant plants lacking S ...[more]

PMID: 39394507

Similar Datasets

Effect of NbRD21 CRISPR knockout on N. benthamiana proteome.

Project description:In this experiment we investigate what effect knocking out the cysteine protease RD21 in N. benthamiana has.

2025-04-24 | PXD063276 | Pride

Using Proteomics for an Insight into the Performance of Activated Sludge

Project description:The performance of a lab-scale wastewater treatment plant during the start-up phase was investigated. A period of varying pH resulted in the loss of ammonium removal efficiency together with a decrease in the specific autotrophic oxygen uptake rate (OUR). From the OUR, it was inferred that the ammonium oxidizing bacteria (AOB) were inhibited by the fluctuation in the pH values. However, OUR alone could not provide the information as to how the AOB were affected at the molecular level. To gain a better insight, shotgun proteomic method was used in this work to quantify the total proteins in the system. Label-free quantification (LFQ) showed that during the time of poor ammonium removal, the marker enzyme hydroxylamine oxidase found in Nitrosomonas sp. was at the lowest LFQ intensity. Based on these results, proteomics has the potential to be used as a monitoring tool. Nevertheless, there are still some restrictions when measuring activated sludge using proteomic method such as the availability of a suitable proteomic database. In this paper, we describe our experience of using publicly available database for identification of activated sludge proteins.

2020-05-18 | PXD014156 | Pride

Ubiquitin-independent disassembly by a p97 AAA-ATPase complex drives PP1 holoenzyme formation

Project description:Protein phosphatase-1 (PP1) dephosphorylates a broad spectrum of substrates to regulate a myriad of cellular processes. This functional diversity relies on the ordered assembly of alternative PP1 holoenzymes. Here, we show that newly synthesized PP1 is first held in an inactive complex by its partners SDS22 and inhibitor-3 (I3) that needs to be disassembled by the p97 AAA-ATPase to promote exchange to substrate specifiers. Unlike p97-mediated degradative processes that require the Ufd1-Npl4 ubiquitin adapters, p97 is targeted to PP1 by p37 and related adapter proteins. Reconstitution with purified components revealed direct interaction of the p37 SEP domain with I3 without the need of ubiquitination, and ATP-driven pulling of I3 into the central channel of the p97 hexamer, which triggers dissociation of I3 and SDS22. Thus, we establish regulatory ubiquitin-independent protein complex disassembly as part of the functional arsenal of p97 and define an unanticipated essential step in PP1 biogenesis that illustrates the molecular challenges of ordered subunit exchange.

2018-10-23 | PXD010750 | Pride

Released peptide from flagellin cleavage by SBT5.2a

Project description:The aim of this experiment is to identify the released peptides from flagellin obtained afte cleavage and processing by SBT5.2

2024-11-02 | PXD056369 | Pride

Elucidating the underlying mechanism behind AtMC1C220A autoimmune phenotype through the study of AtMC1C220A interactors at the microsomal and total fractions.

Project description:Identification of Metacaspase 1 (AtMC1) interacting partners using IP-MS

2025-03-04 | PXD049206 | Pride

Isolation and characterisation of a 14kDa adhesin protein from Rhizobium leguminosarum biovar viciae 3841

Project description:In this project we aimed to identify a 14 kDa adhesin protein from Rhizobium leguminosarum biovar viciae 3841 unsing in gel digestion coupled to LC-MS/MS.

2024-08-19 | PXD029089 | Pride

Effect of Cpd1 on melanoma proteome

Project description:Investigation of the time dependent effect of Cpd1 (small molecule interactor) on the proteome composition of melanoma WM3734 line by label-free quantification using mass spectrometry. The goal was to identify which protein abundances are increased upon treatment with Cpd1. Melanoma cell line WM3734 was treated with Cpd1 and with the control DMSO. Samples were taken at two time points, 24h and 72h.

2022-03-14 | PXD008757 | Pride

ct of PROTAC #89, which targets an endogenously SNAP-tagged clathrin light chain, on the HAP1 cell proteome

Project description:We would like to investigate what effect degradation of the clathrin light chain has on the HAP1 proteome. We therefore tagged the clathrin light chain (CLC) in HAP1 cells endogenously with a SNAP-tag via CRISPaint. The degradation of the SNAP-tagged protein is induced by the addition of a SNAP-PROTAC (PROTAC #89). The proteomes of a short (6 h) and long (24 h) treatment with PROTAC #89 are compared against the corresponding DMSO controls using LFQ.

2025-05-06 | PXD049283 | Pride

Effect of fluvastatin-treatment on the proteome and phosphor-proteome in metastatic pancreatic cancer cells

Project description:Fluvastatin has been identified as novel inhibitors of metastasis in pancreatic cancer. In multiple in vivo and in vitro experiments the compounds phenotypic effects was validated. Since under drug treatment the transcriptomic changes in examined cell lines were rather small, we expected the underlying mechanistic effect to be on the level of the proteome and more importantly the phospho-proteome. To evaluate this assumption we performed label-free full proteome and phosphoproteomics experiments on Fluvastatin treated ASPC1 cells.

2021-11-29 | PXD022345 | Pride

Effect of Bion (BTH treatment) in GST activities on 6-week old A.thaliana plants

Project description:The goal of this project was to investigate GSTs labelled by GST-G probe in non-treated Arabidopsis (Water samples: Click control vs GST-G) or GSTs activated upon Bion treatment of Arabidopsis (Water GST-G vs Bion GST-G).

2024-01-26 | PXD045884 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data