Proteomics

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A new cellular platform for studying autophagy

ABSTRACT: Atg8 proteins play a crucial role in autophagy. In yeast, there is a single Atg8 member, while mammals have six homologs categorized into LC3s and GABARAPs. The GABARAP subfamily consists of GABARAP, GABARAPL1, and GABARAPL2/GATE16 proteins, contributing to various autophagy stages. However, the intricacies among GABARAP proteins are complex and not well-understood. We introduce a new cellular platform to study autophagy, achieved by tagging GABARAP genes with N’-terminal 3xFlag epitopes fused to Clover or mScarletI fluorescent proteins using CRISPR/Cas9. This platform offers robust autophagy examination via flow cytometry, enabling real-time monitoring of GABARAP homologs at single-cell resolution through fluorescent microscopy. The 3xFlag epitope facilitates isolation and characterization of distinct autophagosomes labeled by specific GABARAP homologs. Our platform promises insights into the unique roles of GABARAP homologs in autophagy and possibly other cellular processes.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Yishai Levin  

LAB HEAD: Zvulun Elazar

PROVIDER: PXD049031 | Pride | 2025-05-06

REPOSITORIES: Pride

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Publications

Multiplex genomic tagging of mammalian ATG8s to study autophagy.

Goldin-Azulay Korina K   Fraiberg Milana M   Trofimyuk Olena O   Levin Yishai Y   Reuven Nina N   Kopitman Ekaterina E   Elazar Zvulun Z  

The Journal of biological chemistry 20241019 12

Atg8 proteins play a crucial role in autophagy. There is a single Atg8 isoform in yeast, while mammals have up to seven homologs categorized into LC3s and GABARAPs. The GABARAP subfamily consists of GABARAP, GABARAPL1, and GABARAPL2/GATE16, implicated in various stages along the pathway. However, the intricacies among GABARAP proteins are complex and require a more precise delineation. Here, we introduce a new cellular platform to study autophagy using CRISPR/Cas9-mediated tagging of endogenous  ...[more]

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