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Identification of phosphorylation sites on NAP1/AZI2

ABSTRACT: We observed smearing for the TBK1-adaptor protein NAP1/AZI2 during mitophagy. To verify whether this smearing was due to phosphorylation by TBK1, we performed an in vitro kinase assay and complemented this with the immunoprecipitation of NAP1 from HAP1, whose genetic background (FIP200 knockout) results in hyperactivation of TBK1.

INSTRUMENT(S): Q Exactive HF-X, Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: WeiQiang Chen

LAB HEAD: Sascha Martens

PROVIDER: PXD049184 | Pride | 2024-04-12

REPOSITORIES: Pride

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