Proteomics

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Function of CK2 selectively affects the endoplasmic reticulum and the Golgi apparatus in mTOR-mediated autophagy induction.

ABSTRACT: Selective autophagy of the endoplasmic reticulum, known as ER-phagy, is essential to maintain ER homeostasis. We recently showed that members of the autophagy receptor family FAM134 are regulated by phosphorylation-dependent ubiquitination. In an unbiased screen we had identified several kinases downstream of mTOR with profound impact on ER-phagy flux, upon them ATR and CK2. Inhibition of CK2 by SGC-CK2-1 prevented regulatory ubiquitination of FAM134B and FAM134C upon autophagy activation as well as the formation of high-density FAM134B- and FAM134C-clusters. Here we report on additional resource data of global proteomics upon CK2 and ATR inhibition, respectively. Our data suggests that the function of CK2 is mainly limited to the ER / ER-phagy and Golgi, while ATR inhibition by VE-822 affects the vast majority of organelles / selective autophagy pathways.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Pablo Sanz Martinez  

LAB HEAD: Alexandra Stolz

PROVIDER: PXD049341 | Pride | 2025-03-07

REPOSITORIES: Pride

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20220607_LU_LC2_GT_PS_FamB_625ng_F.msf Msf
20220607_LU_LC2_GT_PS_FamB_625ng_F.msfView Msf
20220607_LU_LC2_GT_PS_FamB_625ng_F.pdResult Other
20220607_LU_LC2_GT_PS_FamB_625ng_F.pdResultView Other
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Publications

Function of CSNK2/CK2 selectively affects the endoplasmic reticulum and the Golgi apparatus in mtor-mediated autophagy induction.

Sanz-Martinez Pablo P   Berkane Rayene R   Stolz Alexandra A  

Autophagy 20240903 2

Selective macroautophagy/autophagy of the endoplasmic reticulum, known as reticulophagy/ER-phagy, is essential to maintain ER homeostasis. We recently showed that members of the autophagy receptor family RETREG/FAM134 are regulated by phosphorylation-dependent ubiquitination. In an unbiased screen we had identified several kinases downstream of MTOR with profound impact on reticulophagy flux, including ATR and CSNK2/CK2. Inhibition of CSNK2 by SGC-CK2-1 prevented regulatory ubiquitination of RET  ...[more]

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