Proteomics

Dataset Information

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Identification of ID4-interacting proteins in cultured undifferentiated spermatogonia

ABSTRACT: ID4 belongs to a family of transcriptional regulators involved in stem cell function. ID family proteins act by binding and inhibiting activity of basic-HLH (bHLH) transcription factors, including E-proteins and class II factors. ID function is also mediated through interaction with non-bHLH proteins. Spermatogonial stem cells (SSCs) are found within a population of undifferentiated spermatogonia in the testis and are essential for the sustained production of sperm over the lifespan. It is reported that ID4 is involved in SSC maintenance but the downstream targets of ID4 in SSCs are unclear. To gain insight into the function of ID4 in SSCs we identified interacting partners in cultures of mouse undifferentiated spermatogonia by immunoprecipitation using a specific ID4 antibody and mass spectrometry analysis.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Type A Spermatogonium

SUBMITTER: Ralf Schittenhelm  

LAB HEAD: Robin Hobbs

PROVIDER: PXD049346 | Pride | 2025-05-06

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
F1RS20210115_1D4.raw Raw
F1RS20210115_IgG.raw Raw
F1RS20210304_WTB_Id4.raw Raw
F1RS20210304_WTB_IgG.raw Raw
F1RS20210304_WTC_Id4.raw Raw
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Publications

Functionally redundant roles of ID family proteins in spermatogonial stem cells.

La Hue M HM   Chan Ai-Leen AL   Hutchinson Ashlee M AM   Su Bianka Y M BYM   Rossello Fernando J FJ   Schittenhelm Ralf B RB   Hobbs Robin M RM  

Stem cell reports 20240926 10

Spermatogonial stem cells (SSCs) are essential for sustained sperm production, but SSC regulatory mechanisms and markers remain poorly defined. Studies have suggested that the Id family transcriptional regulator Id4 is expressed in SSCs and involved in SSC maintenance. Here, we used reporter and knockout models to define the expression and function of Id4 in the adult male germline. Within the spermatogonial pool, Id4 reporter expression and inhibitor of DNA-binding 4 (ID4) protein are found thr  ...[more]

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