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Purification of time-resolved insulin granules reveals proteomic and lipidomic changes during granule aging

ABSTRACT: Endocrine cells employ regulated exocytosis of secretory granules to secrete hormones and neurotransmitters. Secretory granule exocytosis depends on spatio-temporal variables such as proximity to the plasma membrane and age, with newly-generated granules being preferentially released. Despite recent advances, we lack a comprehensive view about the molecular composition of insulin granules and associated changes over their lifetime. Here we report a strategy for the purification of insulin secretory granules of distinct age from insulinoma INS-1 cells. Tagging the granule-resident protein phogrin with a cleavable CLIP tag, we obtain intact fractions of age-distinct granules for proteomic and lipidomic analyses. We find that the lipid composition changes

INSTRUMENT(S): time-of-flight

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Pancreas

DISEASE(S): Diabetes Mellitus

SUBMITTER: Mario Oroshi

LAB HEAD: Matthias Mann

PROVIDER: PXD050087 | Pride | 2024-04-15

REPOSITORIES: Pride

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Small Extracellular Vesicles from Hypoxic Triple-Negative Breast Cancer Cells induce Oxygen-dependent Cell Invasion

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2022-11-01 | PXD035244 | Pride

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2022-04-27 | PXD023904 | Pride

VCF1 is a p97 cofactor promoting recruitment of p97-UFD1-NPL4 to ubiquitylated substrates

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2024-03-14 | PXD043565 | Pride

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2022-07-27 | PXD033293 | Pride

Sildenafil Combined with Controlled Hypothermia Mitigates Microglial Activation After Neonatal Hypoxia-Ischemia in Rat.

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2024-04-30 | PXD045168 | Pride

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2024-01-26 | PXD042470 | Pride

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