Dataset Information

Carrier protein-enabled host cell proteome analysis (CP-HCP) with integrated isobaric tag interference filtering

ABSTRACT: We have created a CP-HCP method to characterise the carrier proteome of a host cell expression system producing recombinant protein biotherapeutic, which can be compared with the testing sample mixture containing trace amounts of host cell proteins against high-background recombinant protein and also a recombinant protein peptide characterisation channel, using TMT labelling.

INSTRUMENT(S):

ORGANISM(S): Escherichia Coli

SUBMITTER: Divyanshi Karmani

LAB HEAD: Dr John Patrick Murphy

PROVIDER: PXD050214 | Pride | 2026-01-12

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	ea17066_20240225013125.mzML	Mzml
	ea17066_20240225013125.pepXML	Pepxml
	ea17066_20240225013125.raw	Raw
	ea17067_20240225032300.mzML	Mzml
	ea17067_20240225032300.pepXML	Pepxml

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Publications

Carrier-Guided Proteome Analysis in a High Protein Background: An Improved Approach to Host Cell Protein Identification.

Karmani Divyanshi D Seifihesar Niloofar N Sultonova Mukhayyo M Blackmore Beau B Paulo Joao A JA Harty Matthew M Murphy J Patrick JP

Journal of proteome research 20241009 11

Many shotgun proteomics experiments are negatively influenced by highly abundant proteins, such as those measuring residual host cell proteins (HCP) amidst highly abundant recombinant biotherapeutic or plasma proteins amidst albumin and immunoglobulins. While western blotting and ELISAs can reveal the presence of specific low abundance proteins from highly abundant background proteins, mass spectrometry approaches are required to define the low abundance protein composition in these scenarios. T ...[more]

PMID: 39382319

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