CryoEM architecture of a near-native stretch-sensitive membrane microdomain

ABSTRACT: Eisosomes, stress-sensitive membrane compartments in yeast, are scaffolded by the BAR-domain proteins Pil1 and Lsp1. We isolated near-native eisosomes as helical filaments of Pil1/Lsp1 lattice bound to plasma membrane lipids and solved their structure by helical reconstruction. From the same near-native eisosome samples, we performed mass spectroscopy to determine the protein content, and the abundance ratio of Pil1/Lsp1.

INSTRUMENT(S):

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Nesli Ece Sen  

LAB HEAD: Prof. Robbie Loewith

PROVIDER: PXD050326 | Pride | 2024-07-18

REPOSITORIES: Pride