Dataset Information

Systematic proteomics analysis of lysine acetylation reveals critical features of renal proteins in kidney calculi formation

ABSTRACT: In this study, we systematically integrated proteome and acetyl proteome (acetylome) approaches to investigate the characteristics of renal proteins in a CaOx crystal rat model. We aimed to understand the pathogenesis of kidney calculi and delineate the landscape of acetylation within kidney calculi, potentially leading to the identification of valuable and novel biomarkers. Using liquid chromatography-tandem mass spectrometry (LC-MS/MS), we analyzed the protein expression profiles and lysine acetylation (Kac) features in kidney tissues obtained from rats with kidney calculi and those without (normal controls). Our results revealed118 downregulated and 129 upregulated proteins. Furthermore, we identified 538 upregulated Kac sites in 258 proteins and 133 downregulated Kac sites in 118 proteins between kidney calculi and paired normal rats. Functional enrichment and protein-protein interaction network analyses revealed that the mitochondria were the most abundant acetylated protein fraction, and metabolic pathways were predominated among the GO and KEGG pathways. Furthermore, the LC-MS/MS findings were verified by immunofluorescence. The study is the first comparative study of Kac modification associated with kidney calculi. These findings offer significant insights into the molecular mechanism underlying the formation and development of renal stones.

INSTRUMENT(S):

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Kidney

SUBMITTER: Qiong Deng

LAB HEAD: Qiong Deng

PROVIDER: PXD050342 | Pride | 2026-02-09

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	XD050LPAc.zip	Other
	XD050LPAc_control1_Slot2-1_1_4308.d.zip	Other
	XD050LPAc_control2_Slot2-2_1_4310.d.zip	Other
	XD050LPAc_control3_Slot2-3_1_4312.d.zip	Other
	XD050LPAc_stone1_Slot2-4_1_4314.d.zip	Other

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Publications

Systematic proteomics analysis of lysine acetylation reveals critical features of renal proteins in kidney calculi formation.

Zhang Shiwei S Wang Zhu Z Jiang Hao H Wang Jieyan J Jin Meiyu M Liang Hui H Deng Qiong Q

PloS one 20260128 1

In this study, we systematically integrated proteome and acetyl proteome (acetylome) approaches to investigate the characteristics of renal proteins in a CaOx crystal rat model. We aimed to understand the pathogenesis of kidney calculi and delineate the landscape of acetylation within kidney calculi, potentially leading to the identification of valuable and novel biomarkers. Using liquid chromatography-tandem mass spectrometry (LC-MS/MS), we analyzed the protein expression profiles and lysine ac ...[more]

PMID: 41604379

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