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Identification of host proteins interacting with the E protein of porcine epidemic diarrhea virus

ABSTRACT: Porcine epidemic diarrhea (PED) is an acute, highly contagious, and high-mortality enterophilic infectious disease caused by the porcine epidemic diarrhea virus (PEDV). PEDV is globally endemic and causes substantial economic losses in the swine industry. The PEDV E protein is the smallest structural protein with high expression levels that interacts with the M protein and participates in virus assembly. However, how the host proteins interact with E proteins in PEDV replication remains unknown. We identified host proteins that interact with the PEDV E protein using a combination of PEDV E protein-labeled antibody co-immunoprecipitation and tandem liquid-chromatography mass-spectroscopy (LC-MS/MS).

INSTRUMENT(S): Q Exactive HF, Thermo Fisher Scientific instrument model

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Cell Culture, Kidney Cell

DISEASE(S): Coronavirus Infectious Disease

SUBMITTER: Qiyuan Kuang

LAB HEAD: Guihong Zhang

PROVIDER: PXD050352 | Pride | 2024-03-05

REPOSITORIES: Pride

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