Dataset Information

Comprehensive Proteomic Profiling of Converted Adipocyte-like Cells from Normal Human Dermal Fibroblast using Data-Independent Acquisition Mass Spectrometry (DIA-MS)

ABSTRACT: We applied direct conversion of normal human dermal fibroblast (NHDF) into adipocyte-like cells using adipogenic cocktail containing IBMX, dexamethasone, insulin, and rosiglitazone and confirmed prominent lipid droplet accumulation in the converted cells for proteomic research

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Fibroblast

SUBMITTER: Muhammad Umar Sohail

LAB HEAD: Sunkyu Choi

PROVIDER: PXD050528 | Pride | 2025-05-13

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20240122_Exploris_DDA_180min_iRT_C_D_1.raw	Raw
	20240122_Exploris_DDA_180min_iRT_C_D_2.raw	Raw
	20240122_Exploris_DDA_180min_iRT_C_D_3.raw	Raw
	20240122_Exploris_DDA_180min_iRT_C_D_4.raw	Raw
	20240122_Exploris_DDA_180min_iRT_C_D_5.raw	Raw

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Publications

Comprehensive Proteomic Profiling of Converted Adipocyte-like Cells from Normal Human Dermal Fibroblasts Using Data-Independent Acquisition Mass Spectrometry.

Choi Sunkyu S Dalloul Rajaa S D RSD Vemulapalli Praveen Babu PB Yousef Sondos S Goswami Neha N Schmidt Frank F

ACS omega 20240909 38

Adipocytes play an important role in the regulation of systemic energy homeostasis and are closely related to metabolic disorders, such as type-2 diabetes and inflammatory bowel diseases. Particularly, there is an increasing need for a human adipocyte model for studying metabolic diseases and obesity. However, utilizing human primary adipocyte culture and stem-cell-based models presents several practical limitations due to their time-consuming nature, requirement for relatively intensive labor, ...[more]

PMID: 39346858

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