Protease Regulation by Subtrate O-Glycosylation

ABSTRACT: In this study, we systematically assessed the fine-tuning of limited proteolysis in the extracellular space by glycosylation, one of the most widely observed PTMs, using cells, and applying the Terminal Amine Labeling of Substrates (TAILS) approach, a quantitative proteomics workflow for the system-wide assessment of protein N termini and protease cleavage events in complex biological samples. In addtion, we complement the study with DIA analysis, PRM and glycoproteomics analysis for further validation of the initial observations.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: Elizabeta Madzharova  

LAB HEAD: Chiara Francavilla

PROVIDER: PXD050581 | Pride | 2024-08-10

REPOSITORIES: Pride