Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Maize plastoglobule and thylakoid proteomes during water deficit and recovery

ABSTRACT: The maize plastoglobule proteome has not been reported in the literature, nor has its dynamic repsonse to water-deficit (i.e. drought) been investigated. To address this shortcoming, we have employed deep bottom-up proteomics of isolated plastoglobules, as well as isolated thylakoids, from 3-week-old maize seedlings experienceing a drought stress treatment. Maize plants were not watered for 17 days, during which a decline in photosynthetic parameters and leaf wilting and tip chlorosis was observed, followed by re-watering from which plants quickly recovered. Plastoglobules and thylakoods were isolated at six different time points during the stress treatment to monitor dynamic changes during entry itno and recovery from the stress. These siz time points are: 7 days without watering (dWW), 12 dWW, 16 dWW, 17 dWW, 1 day after re-watering (dRW), and 3 dRW. The 7 dWW time point represents the basal state prior to imposition of stress (plants are only watered once every 7 days), while the 17 dWW represents the peak stress time point. We find tailored changes to plastoglobule and thylakoid consistent with triggering of specific stress tolerance mechanisms, but no indication of senescence processes were observed. Our results provide the first public report of the maize plastoglobule proteome and its dynamic responses to water-deficit.

INSTRUMENT(S):

ORGANISM(S): Zea Mays (maize)

TISSUE(S): Leaf

SUBMITTER: Peter Lundquist

LAB HEAD: Peter K

PROVIDER: PXD050599 | Pride | 2025-10-17

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	File_list.xlsx	Xlsx
	MaizeGDB_Zea_mays.AGPv3.22.pep.all.fa	Other
	MaxQuant_v1.5.5.1.zip	Other
	PG_MBR.zip	Other
	Thy_MBR.zip	Other

Items per page:

1 - 5 of 54

Similar Datasets

Arabidopsis Plastoglobule Proteome with ABC1 atypical kinase mutants

Project description:Quantitative proteomics were performed on tryptic digests of proteins purified from the thylakoid and plastoglobules of Arabidopsis thaliana. Wild type was compared to null mutants of ABC1 atypical kinases, specifically abc1k1, abc1k3, and abc1k6.

2020-02-06 | PXD017380 | Pride

Genome-wide transcriptome analysis of two maize inbred lines under drought stress during the seedling stage

Project description:To understand the transcriptome changes during drought tolerance in maize, the drought-tolerant line Han21 and drought-sensitive line Ye478, which show substantial differences in drought tolerance at the seedling stage, were selected for this study. Using the GeneChip Maize Genome Arrays, we applied genome-wide gene expression analysis to the two genotypes under gradual drought stress and re-watering. We identified 2172 common regulated transcripts in both lines under drought stress, with 1084 common up-regulated transcripts and 1088 common down-regulated transcripts. Among the 2172 transcripts, 58 potential protein kinases and 117 potential transcription factors were identified. The potential components of the ABA signaling pathway were identified from the common regulated transcripts. We also identified 940 differentially regulated transcripts between the two lines. Among the 940 transcripts, the differential expression levels of 29 transporters and 15 cell wall-related transcripts may contribute to the different tolerances of the two lines. Additionally, we found that the drought-responsive genes in the tolerant Han21 line recovered more quickly when the seedlings were re-watered, and 311 transcripts in the tolerant Han21 line were exclusively up-regulated at the re-watering stage compared to the control and stress conditions. Our study provides a global characterization of two maize inbred lines during drought stress and re-watering and will be valuable for further study of the molecular mechanisms of drought tolerance in maize. In two independent experiments, we generate maize gene expression profiles during drought stress and re-watering through comparing genome-wide expression patterns of drought stress treatment and re-watering treatment by using 17,555 Affymetrix maize whole genome array.

2010-06-16 | E-GEOD-16567 | biostudies-arrayexpress

Genome-wide transcriptome analysis of two maize inbred lines under drought stress during the seedling stage

2010-06-03 | GSE16567 | GEO

Plastoglobules are a biosynthetic platform contributing to carotenoid accumulation during tomato fruit maturation

Project description:Chloroplasts and chromoplasts of tomato fruits contain lipid droplets known as plastoglobules (PG). Recent studies have shown that PG act as a metabolic sink to store triacylglycerol (TAG), carotenoids, vitamin E, and K as well as other lipophile compounds. In addition, it has been shown that proteins associated with PG contribute to prenyl lipid metabolic pathways and homeostasis. Proteome studies in Arabidopsis and bell pepper, have demonstrated that PG contains a specific proteome containing around 30 proteins. So far, the tomato PG proteome particularly that of red fruit (chromoplast) has not yet been established. We have investigated the chromoplast PG proteome and metabolome and identified 17 new candidate proteins of chromoplast PG with known PG proteins. Interestingly, most of the carotenoid biosynthetic pathway enzymes such as PSY‐1, PDS, ZDS, CRTISO, and β‐LYC are enriched in chromoplast PG compared with chloroplast PG. In this study, we propose a model of chromoplast PG acting as a carotenoid biosynthesis platform.

2023-03-11 | PXD035763 | Pride

Quantitative proteomics of isolated A. thaliana plastoglobules from wild-type, abc1k6-1 and Comp3

Project description:The quantitative proteomes of isolated plastoglobules have been determined from A. thaliana leaf chloroplasts at 4 weeks of growth using tandem mass spectrometry with label-free quantification. A comparison of three genotypes has been performed, namely i) the Col-0 wild type ecotype, ii) a T-DNA insertion mutant disrupting the plastoglobule-localized ABC1K6 protein (abc1k6-1) that is delayed in transition to reproductive growth, and iii) a complementation line (Comp3) that restores the timely progression of development with the ABC1K6 wild-type protein sequence tagged C-terminally with GFP in the abc1k6-1 background. The levels of ten plastoglobule proteins were found to be effected by the loss of ABC1K6. Of particular note, ABC1K7 was strongly depleted in abc1k6-1 and over-accumulated in Comp3, suggesting it may be stabilized by ABC1K6.

2022-03-02 | PXD030698 | Pride

Arabidopsis thylakoid domains proteome

Project description:The thylakoid membrane in land plants is organized into an appressed domain (grana), enriched in photosystem (PS) II, and a non-appressed domain (stroma lamellae) enriched in PSI. This ultrastructure ensures control over excitons spillover from PSII to PSI. The bulky machinery required for the repair and biogenesis of PSII and other proteins of the stacked, appressed domain is located in the non-appressed membranes. Thus, the connecting domain (CD) between grana and stroma lamellae, also known as the grana margins (GM), is the key player in both the structural and functional integrity of the photosynthetic machinery. To elucidate the properties and composition of the GM proteome, we have analyzed the different thylakoid domains isolated from membranes in native conformation as well as from thylakoids artificially unstacked to induce a homogeneous protein composition. We show that the composition of the CD is strictly distinct from that of the highly curved structures (the curvature) present at the periphery of grana discs, which are not physically connected to the stroma lamellae. Furthermore, a detailed map of the composition, distribution and interaction of the protein complexes involved in thylakoid membrane function is provided for the grana, CD, curvature and stroma lamellae domains.

2025-02-11 | PXD046544 | Pride

Thylakoids of Picea abies - The unique photosynthetic apparatus of Pinaceae - Analysis of photosynthetic complexes in Norway spruce (Piceaabies)

Project description:Analysis of the subunits composition of the thylakoids protein complexes in Picea abies (Norway spruce) by means of two-dimensional large-pore Blue-Native/sodium dodecyl sulfate-polyacrylamide gel electrophoresis (2D lpBN/SDS-PAGE) and in-gel tryptic digestion of single spots.

2019-04-02 | PXD010071 | Pride

Plastoglobules BN-PAGE experiment (Arabidopsis PGs under unstress and high light conditions)

Project description:Proteome comparison of isolated plastoglobules (PGs) from Arabidopsis thaliana (Col-0 ecotype) under unstress (UNS) and high light (HL) stress conditions. For both conditions, PGs were isolated from three independent set of plants each (i.e. three for UNS and three for HL). PGs were isolated from the plants when they were 4 weeks old under long day photoperiod (16 hours of light and 8 darkness). The light stress was applied to the designated set of plants 5 days prior the completion of the 4 weeks by shifting the light intensity from 120 uE to 500 E. The PGs were solubilized with 1.1 % n-Dodecyl--D-Maltoside and separated in a Blue-Native gel. After the electrophoretic separation, gel lanes were cut and further divided into 10 independent slices corresponding to different molecular weights. These gel slices were subjeted to in-gel digestion with trypsin and the resulting peptides labeled with TMT reagents. Labeled peptides were mixed 1:1 and purified by solid phase extraction using c18 StageTips. Eluted peptides were sprayed into a ThermoScientific Q-Exactive HF-X mass spectrometer. Survey scans were taken in the Orbi trap (120000 resolution, determined at m/z 200) and the top 20 ions in each survey scan are then subjected to automatic higher energy collision induced dissociation (HCD) with fragment spectra acquired at 45000 resolution. Additionally, PG samples that were not separated in a Blue-native gel were trypsin digested and subjected for mass-spectrometry analysis as previously described.

2021-04-13 | PXD019735 | Pride

Thylakoid protein phosphorylation in Physcomitrella patens

Project description:Physcomitrella patens is a model plant representing the group of mosses, which diverged from vascular plant ancestors early after land colonization. To investigate the unique characteristic of the composition and post-translational regulation of Physcomitrella patens photosynthetic apparatus, we have separated the thylakoids protein complexes in their subunits composition by means of two-dimensional large-pore Blue-Native/sodium dodecyl sulfate-polyacrylamide gel electrophoresis (2D lpBN/SDS-PAGE), followed by protein in-gel tryptic digestion of single spots. To identify the kinase responsible for phosphorylation of the major thylakoid protein complex photosystem II, a mutant line lacking the ortholog of kinase STN8 in the model angiosperm Arabidopsis thaliana has been generated and the identified thylakoid protein phosphorylation pattern have been compared with the WT moss.

2019-05-08 | PXD012501 | Pride

B73 maize total leaf proteome under a heat stress time-course

Project description:A heat stress treatment was imposed on B73 inbred maize three weeks after germination by increasing ambient air temperatures in the greenhouse from 25C to 30C for one day followed by 40C for 7 days, before a recovery phase at 25C for 3 days. We developed a broad-level view of heat stress effects by determining the quantitative total leaf proteome at the reference unstressed time-point (0 days heat stress; 25C), the peak heat stress time-point (7 days heat stress), and the recovery time point (3 days heat recovery).

2025-10-17 | PXD061470 | Pride