Dataset Information

Paired Proteomic Analysis of Sun-Exposed Skin of Professional Drivers Reveals altered abundance of Keratins, S100A14, Hemoglobin, and F-box Proteins.

ABSTRACT: Professional drivers represent an ideal cohort for investigating the effects of solar radiation on skin due to their unique, asymmetric exposure to sun, a consequence of vehicle window orientations. Consequently, one side of the face is naturally subjected to more solar radiation, resulting in uneven sunlight exposure. This unique exposure scenario supports a paired experimental design for precise within-individual comparisons, crucial for assessing sun exposure's impact on skin health. Leveraging this approach, our study reveals sun-induced overexpression of proteins linked to photoaging through paired proteomic analysis, providing novel insights into the skin's adaptive responses to chronic solar exposure. Initially, our research focused on a dataset from ten male professional drivers, identifying a set upregulated proteins in sun-exposed skin compared to the less exposed side of the face. To validate these findings, we extended our investigation to a new cohort of seven female bus drivers. Our motivation in switching genders was for assessing the robustness of our initial findings, regardless of sex, and also for understanding the broader implications of our results for photodermatology. To facilitate this detailed analysis, we developed specialized software that enables precise paired proteomic analysis, significantly enhancing the robustness and clarity of our findings. Our results shortlisted keratins, S100A14, and F-box proteins—by remaining consistently overexpressed in sun-exposed skin—and hemoglobin subunit beta as downregulated across both cohorts. Our findings underscore the potential of proteomic techniques in advancing our understanding of the molecular dynamics of photoaging and highlight the value of selecting cohorts with specific exposure characteristics.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Skin

SUBMITTER: Marlon D M Santos

LAB HEAD: Paulo Costa Carvalho

PROVIDER: PXD050746 | Pride | 2025-05-06

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20231025_183523_Amanda_1_1.raw	Raw
	20231025_183523_Amanda_1_1.yada.ctxt	Other
	20231025_183523_Amanda_1_2.raw	Raw
	20231025_183523_Amanda_1_2.yada.ctxt	Other
	20231025_183623_Amanda_2_1.raw	Raw

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Publications

Paired proteomic analysis reveals protein alterations in sun-exposed skin of professional drivers : <sup>1</sup>Laboratory for structural and Computational Proteomics, Carlos Chagas Institute, Fiocruz, Paraná, Brazil.

Camillo-Andrade Amanda C AC Sales Lucas A LA Fischer Juliana S G JSG Duran Rosario R Santos Marlon D M MDM Carvalho Paulo C PC

Scientific reports 20250331 1

Professional drivers represent an ideal cohort for investigating the effects of solar radiation on skin due to their unique, asymmetric exposure to sun, a consequence of vehicle window orientations. Consequently, one side of the face is naturally subjected to more solar radiation, resulting in uneven sunlight exposure. This scenario supports a paired experimental design for precise within-individual comparisons, crucial for assessing sun exposure's impact on skin health, including signs of aging ...[more]

PMID: 40164647

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Project description:We here present a novel subtype of fibroblasts isolated from sun-exposed skin of elderly donors and characterized by its prominent and stable myo-chondrocyte-type differentiation. As we depict a similar chondrocyte-type expression pattern at sites of solar elastosis in human skin, we propose that this fibroblasts phenotype develops in the course of massive and perseverative UV-dependent damage as part of the process terminating in the most severe appearance of photoaging - solar elastosis. Along with the abnormal expression profile, these “old” fibroblasts generate a chondrocyte-type matrix with a layer of myofibroblast-differentiation in close vicinity to the epidermis, when propagated as skin equivalents (SEs) in 3D organotypic cultures. Importantly, these fibroblasts are strongly reduced in their ability to support epidermal growth and differentiation, allowing for rapid/premature epidermal atrophy. Having identified TGFß as an important player in this scenario, we show that the addition of TGFß to normal young fibroblast induces while inhibition of TGFß to the old fibroblast abolishes the myo-chondrogenic expression profile. Accordingly, inhibiting TGFß in the in vivo-like SEs, not only abrogates the aberrant phenotype but allows these fibroblasts to regain their normal mutual interaction with the epidermal keratinocytes and to support epidermal regeneration and longevity. Further, we provide evidence for a role of chronic solar UV radiation in initiating chondrogenic gene expression in young fibroblasts. Concomitantly, we show however, that the same UV treatment abolishes myofibroblast differentiation in the SEs with the “old” chondrogenic fibroblasts, and that this selective inhibition is already sufficient to restore the fibroblasts’ ability for successfully supporting epidermal growth and differentiation. From this it is tempting to suggest, that besides all damaging potential, “mild” solar UV radiation may exert also beneficial effects, e.g. in promoting activation of photoaged skin.

Dataset Information

Paired Proteomic Analysis of Sun-Exposed Skin of Professional Drivers Reveals altered abundance of Keratins, S100A14, Hemoglobin, and F-box Proteins.

Dataset's files

Publications

Paired proteomic analysis reveals protein alterations in sun-exposed skin of professional drivers : <sup>1</sup>Laboratory for structural and Computational Proteomics, Carlos Chagas Institute, Fiocruz, Paraná, Brazil.

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Dataset Information

Paired Proteomic Analysis of Sun-Exposed Skin of Professional Drivers Reveals altered abundance of Keratins, S100A14, Hemoglobin, and F-box Proteins.

Dataset's files

Publications

Paired proteomic analysis reveals protein alterations in sun-exposed skin of professional drivers : &lt;sup&gt;1&lt;/sup&gt;Laboratory for structural and Computational Proteomics, Carlos Chagas Institute, Fiocruz, Paraná, Brazil.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Paired proteomic analysis reveals protein alterations in sun-exposed skin of professional drivers : <sup>1</sup>Laboratory for structural and Computational Proteomics, Carlos Chagas Institute, Fiocruz, Paraná, Brazil.