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DIA-based protein quantification of mouse oocytes from five developmental stages.

ABSTRACT: The oocytes in 6 replicates from five stages were analyzed using DIA-based protein quantification technology.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Egg, Oocyte

SUBMITTER: yue wang

LAB HEAD: Xuejiang Guo

PROVIDER: PXD050840 | Pride | 2025-01-29

REPOSITORIES: Pride

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			Action	DRS
	P10_DIA_R1_20230105.raw	Raw
	P10_DIA_R2_20230105.raw	Raw
	P10_DIA_R3_20230106.raw	Raw
	P10_DIA_R4_20230107.raw	Raw
	P10_DIA_R5_20230108.raw	Raw

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1 - 5 of 35

Publications

Integrative Omics Reveals the Metabolic Patterns During Oocyte Growth.

Zhang Xiang X Ge Juan J Wang Yue Y Chen Minjian M Guo Xuejiang X Zhu Shuai S Wang Hui H Wang Qiang Q

Molecular & cellular proteomics : MCP 20241015 11

Well-controlled metabolism is associated with high-quality oocytes and optimal development of a healthy embryo. However, the metabolic framework that controls mammalian oocyte growth remains unknown. In the present study, we comprehensively depict the temporal metabolic dynamics of mouse oocytes during in vivo growth through the integrated analysis of metabolomics and proteomics. Many novel metabolic features are discovered during this process. Of note, glycolysis is enhanced, and oxidative phos ...[more]

PMID: 39414232

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Quantitative proteomics analysis of RPL12 mutant and WT mouse oocytes

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Gene Expression Profiles in Immature and In vitro matured bovine Oocytes

Project description:In cattle, almost all fully grown vesicle stage oocytes (GV) have the ability to resume meisos, develop to Metaphase II stage (MII), support fertilization and progress through the early embryonic cycles in vitro. Yet without intensive selection, the majority fail to develop to the blastocyst stage. Using the Affymetrix Bovine Genome Array, global mRNA expression analysis of immature (GV) and in vitro matured (IVM) bovine oocytes was carried out to characterize the transcriptome of the bovine oocyte and to identify the key pathways associated with oocyte meiotic maturation and developmental potential. Immature and in vitro matured bovine oocytes were collected for RNA extraction and hybridization on Affymetrix GeneChip Bovine Genome array. Careful removal of cumulus and selection of oocytes was carried out under the stereo microscope in order to examine the actual cumulus-free temporal oocyte gene expression profiles. Immature oocytes at time 0 h and in vitro matured oocytes at 24 h were collected for analysis.

2011-04-25 | E-GEOD-23449 | biostudies-arrayexpress

Mammalian oocytes store proteins in cytoplasmic lattices

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2023-10-23 | PXD038575 | Pride

Transcription profiling of mouse oocytes in different development stages to study molecular events during oocyte development.

Project description:oocytes were collected from ovary by follicle stage. Targets from four biological replicates of each were generated and the expression profiles were determined using Affymetrix MOE430A and B. Experiment Overall Design: 4 biological replicates were analyzed

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Global gene analysis of oocytes from early stages in human folliculogenesis

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Transcriptome profile of oocytes expressing a catalytically inactive knock-in allele of Ago2

Project description:The RNase III enzyme DICER generates both microRNAs (miRNAs) and endogenous short interfering RNAs (endo-siRNAs). Dicer deletion in mouse oocytes leads to female infertility due to defects during meiosis I. Because miRNA function is suppressed in mouse oocytes, it has been proposed that endo-siRNAs may have a role during female meiosis. By utilizing a catalytically inactive knock-in allele of AGO2 specifically in oocytes, we disrupt the function of siRNAs and analyze the transcriptome of these oocytes in comparison with Ago2 null, and Dicer null oocytes. Germinal vesicle-intact, full-grown oocytes were collected from eCG-primed females expressing a catalytically inactive knock-in allele of Ago2 (Ago2 ADH) specifically in oocytes. Oocytes were also collected from Ago2 null and Ago2 fl/fl oocytes, as well as Dicer wild-type and null mice. Oocytes were freed of attached cumulus cells by pipetting. Twenty oocytes collected from 3 different mice were used for high-throughput RNA sequencing. Three replicates for all genotypes except Ago2 null (two). The mouse background strain is "mostly" C57BL6, i.e., it is a cross between 3 different transgenic mice of different strains.

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Quantitative proteomic analysis of oocytes from high-fat diet (HFD)-fed and normal diet mice

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Microarray profiling of YTHDF2 deficient mouse oocytes

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