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Vagus Nerve Stimulation enhances remyelination and decreases innate neuroinflammation in a Multiple Sclerosis rodent model.

ABSTRACT: This study investigated the effect of Vagus Nerve Stimulation (VNS) on innate neuroinflammation and remyelination in lysolecithin (LPC) induced demyelination, a preclinical model for Multiple Sclerosis (MS). In a first experiment (demyelination experiment), LPC was injected in the corpus callosum of 33 Lewis rats, inducing a demyelinated lesion, and rats were treated with either continuously-cycled VNS (cVNS) or one-minute per day VNS (1minVNS) or sham VNS, from two days before the injection until three days post-injection (dpi), when they were killed for immunohistochemistry and proteomics analysis. This timepoint corresponded with a demyelinated lesion and peak inflammation. In a second experiment (remyelination experiment), 13 rats were analogously treated with either cVNS or sham from two days before LPC injection until 11 dpi, when they were killed for tissue prelevation for immunohistochemistry and proteomics. This timepoints corresponded with partial remyelination of the lesion. For proteomics analysis, 20 rats were randomly selected, namely five cVNS and five sham rats of the demyelination experiment, and five cVNS and five sham rats of the remyelination experiment.

INSTRUMENT(S): timsTOF SCP

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Brain

SUBMITTER: Sara Dufour

LAB HEAD: Helen Bachmann

PROVIDER: PXD050858 | Pride | 2024-05-10

REPOSITORIES: Pride

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			Action	DRS
	Sham.zip	Other
	T03219_EvoAurEl3_20SPDDIAPASEF-PRC-6146_1_S1-F1_1_3564.d.zip	Other
	T03220_EvoAurEl3_20SPDDIAPASEF-PRC-6146_2_S1-F2_1_3565.d.zip	Other
	T03221_EvoAurEl3_20SPDDIAPASEF-PRC-6146_3_S1-F3_1_3566.d.zip	Other
	T03222_EvoAurEl3_20SPDDIAPASEF-PRC-6146_4_S1-F4_1_3567.d.zip	Other

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