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Hydrogen-deuterium exchange mass spectrometry analysis of the CDK2-cyclin A-CDC25A complex

ABSTRACT: The cell division cycle 25 phosphatases CDC25A, B and C regulate cell cycle transitions by dephosphorylating residues in the conserved glycine-rich loop of cyclin-dependent protein kinases (CDKs) to activate CDK activity. Here, we present HDX-MS data to complement the cryogenic-electron microscopy (cryo-EM) structure of CDK2-cyclin A in complex with CDC25A, providing a detailed structural analysis of the overall complex architecture and key protein-protein interactions that underpin this 86 kDa complex. This work improves our understanding of the roles of CDC25 phosphatases in CDK regulation and may inform the development of CDC25-targeting anticancer strategies.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Antonio Calabrese

LAB HEAD: Jane Endicott

PROVIDER: PXD050866 | Pride | 2024-05-29

REPOSITORIES: Pride

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			Action	DRS
	both.DnX	Other
	cdc25a.DnX	Other
	fbsjrau_200817I_HDX_svitlana_cdc25a_03.raw.zip	Raw
	fbsjrau_200817I_HDX_svitlana_cdc25a_05.raw.zip	Raw
	fbsjrau_200817I_HDX_svitlana_cdc25a_07.raw.zip	Raw

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