Quantitative assessment of microbiomes using ultra-fast MS/MS-free metaproteomics: model 2

ABSTRACT: This study presents an adaptation of the novel ultrafast MS1-based method called DirectMS1 to characterization of the single microorganisms and microbiomes. Using DirectMS1 we demonstrate the blind identification (with no prior knowledge of the bacterias in the sample) of a single bacterial species and microbial compositions using two-step database search against the microbial part of SwissProt and TrEMBL (62Gb database size), the evaluation of fold changes in biomass of microorganisms between the metaproteomic samples, and the assessment of metabolic activity of Rhodococcus species responding to a change in growth conditions and cold shock.

INSTRUMENT(S):

ORGANISM(S): Rhodococcus Qingshengii Rhodococcus Opacus Gordonia Polyisoprenivorans Gordonia Alkanivorans Rhodococcus Erythropolis

SUBMITTER: Tomiris Kusainova  

LAB HEAD: Dr Irina A. Tarasova

PROVIDER: PXD050887 | Pride | 2026-08-07

REPOSITORIES: Pride